Dye pollution in the aquatic environment is one of the main environmental and public health issue nowadays. Biosorptive removal of such dyes by biocomposite materials have recently emerged a good potential. For this purpose a novel microbial cell-immobilized Platanus orientalis leaf tissue (NSPOL) was prepared through a passive immobilization, in this study. Its bioremoval potential was investigated for Reactive Red 198 (RR198) and Reactive Yellow 2 (RY2) dyes at different conditions, including pH, biomaterial dosage, contact time and flow rate. Biosorption of RR198 and RY2 by NSPOL was found to be a fast process. Biosorption data were well predicted with the pseudo-second-order kinetic model for both dyes. Decolorization patterns fitted well with Langmuir isotherm model. Maximum monolayer biosorption capacities were recorded as 51.12 and 37.59 mg g⁻¹ for RY2 and RR198, respectively. IR and SEM analysis revealed the biosorption of dyes onto biocomposite. Regeneration experiments showed that the biosorption performance of NSPOL was consistent towards RR198 and RY2 removal upto 5 recycles. After 5 biosorption-desorption cycles, the regeneration efficiency of NSPOL was still higher than 98 % for RY2 dye. Overall, batch and column studies indicated that suggested biocomposite may be a good and economic alternative for the removal of reactive azo dye contamination from aquatic media. Possible mechanism for biosorptive dye removal may be H-bonding, π−π and electrostatic interactions.

水生环境中的染料污染是当今主要的环境和公共卫生问题。通过生物复合材料生物吸附去除此类染料近来已显示出良好的潜力。为此，一种新型的微生物固定化侧柏在这项研究中，通过被动固定制备了叶片组织（NSPOL）。在不同条件下，包括pH，生物材料用量，接触时间和流速，研究了活性红198（RR198）和活性黄2（RY2）染料的生物去除潜力。发现NSPOL对RR198和RY2的生物吸附是一个快速的过程。两种染料的伪二级动力学模型可以很好地预测生物吸附数据。脱色模式非常适合Langmuir等温线模型。记录的最大单层生物吸附容量为51.12和37.59 mg g ^-1分别用于RY2和RR198。红外和扫描电镜分析显示染料在生物复合材料上的生物吸附。再生实验表明，NSPOL的生物吸附性能在RR198和RY2去除多达5个循环中是一致的。经过5个生物吸附-解吸循环，NSPOL对RY2染料的再生效率仍高于98％。总的来说，批处理和色谱柱研究表明，建议的生物复合物可能是从水生介质中去除反应性偶氮染料污染的良好且经济的选择。生物吸附染料去除的可能机理可能是H键，π-π和静电相互作用。