This study investigates the degradation of norfloxacin by using UV combined with hydrogen peroxide (UV/H₂O₂) with different cations and anions. The inhibition effect order of cations for degradation of norfloxacin was Cu²⁺ > Ca²⁺ > Mg²⁺, attributed to the forms of the coordination compounds between norfloxacin and the cations. The inhibition effect order of anions for degradation of norfloxacin was CO₃²⁻ > NO₃⁻ > SO₄²⁻, attributed to the ability and efficiency of the anions to quench ·OH or H₂O₂. The order of the reactive oxygen species (ROS) contribution rate to the degradation of norfloxacin under UV/H₂O₂ was ·OH (72.56%) > ¹O₂ (24.24%) > other ROS (3.20%) based on the trapping experiments and electron spin resonance (ESR) measurements. There were at least 8 kinds of intermediate products, with charge-to-mass ratio (m/z) of 294, 267, 333a, 333b, 347, 335, 351 and 317, which were formed by attacks at the piperazine ring and F atom active sites. The F atom was replaced with -OH via a substitution reaction, resulting in P1 (m/z = 317) formation. The inhibition effect of P1 on DNA gyrase A was stronger than that of norfloxacin due to the binding free energy (the binding free energy of P1-DNA gyrase A was -12.8457 kJ/mol, and the binding free energy of norfloxacin-DNA gyrase A was -11.8488 kJ/mol).

本研究研究了紫外线与过氧化氢（UV / H ₂ O ₂）和不同阳离子和阴离子的组合对诺氟沙星的降解作用。阳离子对诺氟沙星降解的抑制作用顺序为Cu ²⁺ > Ca ²⁺ > Mg ²⁺，这归因于诺氟沙星与阳离子之间的配位化合物形式。阴离子对诺氟沙星降解的抑制作用顺序为CO ₃ ²⁻ > NO ₃ ⁻ > SO ₄ ²⁻，这归因于阴离子淬灭·OH或H ₂ O ₂的能力和效率。。在紫外/ H ₂ O ₂下，活性氧对诺氟沙星降解的贡献率顺序为·OH（72.56％）> ¹ O ₂（24.24％）>其他ROS（3.20％）（基于捕集）实验和电子自旋共振（ESR）测量。至少有8种中间产物，其质荷比（m / z）为294、267、333a，333b，347、335、351和317，它们是通过对哌嗪环和F的攻击而形成的原子活性位点。通过取代反应将F原子替换为-OH，得到P1（m / z = 317）编队。P1对DNA促旋酶A的抑制作用强于诺氟沙星，这是由于其结合自由能（P1-DNA促旋酶A的结合自由能为-12.8457 kJ / mol，而诺氟沙星与DNA促旋酶A的结合自由能为-11.8488kJ / mol）。