Abstract A catalyst consisting of carbon nanotube, 2,2,6,6-Tetramethylpiperidine 1-oxyl (TEMPO), polyacrylic acid (PAA), polyethylenimine (PEI), and glucose oxidase (GOx) (CNT/[TEMPO-PAA]/PEI/GOx) is developed to improve the stability and sensitivity of mediator based glucose sensor. In this catalyst, TEMPO is bonded with PAA to prevent the leaching out of TEMPO, which plays a crucial role in increasing the glucose sensitivity by instilling charges in it. The TEMPO-PAA composite is then linked to other components by electrostatic force. To confirm the chemical structure of the catalyst, its chemical bonds and surface charge are investigated by Fourier-transform infrared spectroscopy and zeta potential. When the stability and glucose sensitivity are measured, this catalyst preserves 80% of its initial catalytic activity for one week although other similar catalysts show far worse stability. In addition, the linear relationship of glucose concentration and reactivity of glucose oxidation shown in 1–10 mM glucose range that corresponds to actual blood glucose concentration of human being indicates that this catalyst can induce excellent glucose sensitivity. In terms of selectivity, this catalyst reacts with blood glucose highly selectively, whereas this is not reacted with other eight different saccharides.

中文翻译：

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多层叠层通孔催化剂测量介体型酶葡萄糖传感器稳定性和灵敏度的研究

摘要 一种由碳纳米管、2,2,6,6-四甲基哌啶 1-氧基 (TEMPO)、聚丙烯酸 (PAA)、聚乙烯亚胺 (PEI) 和葡萄糖氧化酶 (GOx) (CNT/[TEMPO-PAA]/ PEI/GOx) 的开发是为了提高基于介质的葡萄糖传感器的稳定性和灵敏度。在这种催化剂中，TEMPO 与 PAA 结合以防止 TEMPO 浸出，这在通过向其中注入电荷来提高葡萄糖敏感性方面起着至关重要的作用。TEMPO-PAA 复合材料然后通过静电力连接到其他组件。为了确认催化剂的化学结构，通过傅里叶变换红外光谱和zeta电位研究其化学键和表面电荷。当测量稳定性和葡萄糖敏感性时，尽管其他类似催化剂的稳定性要差得多，但这种催化剂可以在一周内保持其初始催化活性的 80%。此外，在 1-10 mM 葡萄糖范围内显示的葡萄糖浓度与葡萄糖氧化反应性的线性关系对应于人类的实际血糖浓度，表明该催化剂可以诱导出色的葡萄糖敏感性。在选择性方面，这种催化剂与血糖反应具有高度选择性，而不会与其他八种不同的糖类反应。葡萄糖浓度和葡萄糖氧化反应性的线性关系显示在 1-10 mM 葡萄糖范围内，对应于人类的实际血糖浓度，表明该催化剂可以诱导极好的葡萄糖敏感性。在选择性方面，这种催化剂与血糖反应具有高度选择性，而不会与其他八种不同的糖类反应。葡萄糖浓度和葡萄糖氧化反应性的线性关系显示在 1-10 mM 葡萄糖范围内，对应于人类的实际血糖浓度，表明该催化剂可以诱导极好的葡萄糖敏感性。在选择性方面，这种催化剂与血糖反应具有高度选择性，而不会与其他八种不同的糖类反应。