Membrane blocking is of great importance to nutrients transfer and illumination in attached cultivation of algae, which subsequently affects the biomass and nutrients production of algal biofilm. Accordingly, a vertical-algal-biofilm (VAB) with cellulose acetate (CA) membranes with pore size of 0.45, 1.2, 2.0, and 3.0 μm is settled to study the effect of membrane blocking on attached cultivation of microalgae. According to the results, the algae production is independent of the roughness, hydrophilicity, and surface free energy of membranes because the VAB is inoculated via filtration-on method in this work. Nevertheless, the algae productivity of first-cultivation on VABs increases to 5.02 g/m².day when the pore size of CA membrane increases to1.2 μm, while it decreases to 4.5 g/m².day on VABs with CA-3.0 due to the complete blocking of CA membranes by algae cells. The growth rates of first-cultivation and re-cultivation are well fitted by a logistic model and a linear model, respectively. The total lipids contents of microalgae on different CA membranes are affected by pore size due to the variations of nutrients transfer resistance and light intensity under different membrane blocking models. A maximum total lipids content of algae biomass cultivated on VABs with CA-1.2 is about 23.74% due to the nitrogen starvation and high illumination intensity under intermediate blocking model. Therefore, the attached cultivation of algae on VABs under intermediate blocking model is much favorable for algal biomass and lipid production.

在藻类的附着培养中，膜阻滞对于养分的转移和光照非常重要，随后会影响藻类生物膜的生物量和养分的产生。因此，沉降具有孔径为0.45、1.2、2.0和3.0μm的醋酸纤维素（CA）膜的垂直藻类生物膜（VAB），以研究膜封闭对微藻附着培养的影响。根据结果​​，藻类的生产与膜的粗糙度，亲水性和表面自由能无关，因为在这项工作中，VAB是通过过滤法接种的。但是，当CA膜的孔径增加到1.2μm时，在VAB上进行首次培养的藻类生产率提高到5.02 g / m ² .day，而到4.5 g / m ^{2时降低。}由于藻类细胞完全阻断了CA膜，因此在带有CA-3.0的VAB上使用了一天。逻辑模型和线性模型分别很好地拟合了初次培养和再培养的增长率。在不同的膜阻滞模型下，不同营养膜的养分抗性和光强度的变化会影响孔径对微藻藻类总脂质含量的影响。由于在中间阻断模型下氮饥饿和高光照强度，在CA-1.2的VAB上培养的藻类生物质的最大总脂质含量约为23.74％。因此，在中间阻断模式下，在VABs上进行藻类的附着培养非常有利于藻类生物量和脂质的生产。