As a preliminary step to characterize genes encoding ATP-Binding-Cassette (ABC) proteins, we cloned a gene encoding an ABC transporter from P. occitanis using a PCR based approach followed by a genomic library screening and by additionally using whole genome sequencing results. The encoded protein has high similarity to the pleiotropic drug resistance protein subfamily members. Analysis of the cloned sequence revealed the presence of Walker A, Walker B and the ABC signature motifs at the nucleotide binding domains. Molecular docking resulted in predicting the most stable complex between the gene-encoding protein and cycloheximide. The southern blot results indicate that the gene is present as a single copy in the P. occitanis genome. The genome-scale identification of the PoABC superfamily members led to the characterization of 58 putative proteins divided into five subfamilies including: 12 ABCB, 24 ABCC, 1 ABCE, 5 ABCF, 15 ABCG, and of which 51 contain trans-membrane domains.

作为表征编码 ATP 结合盒 (ABC) 蛋白的基因的初步步骤，我们使用基于 PCR 的方法从P. occitanis克隆编码 ABC 转运蛋白的基因，然后进行基因组文库筛选并另外使用全基因组测序结果。编码的蛋白质与多效耐药蛋白亚家族成员具有高度相似性。对克隆序列的分析揭示了在核苷酸结合域中存在 Walker A、Walker B 和 ABC 特征基序。分子对接导致预测基因编码蛋白和放线菌酮之间最稳定的复合物。Southern 印迹结果表明该基因在P. occitanis中以单拷贝形式存在基因组。PoABC 超家族成员的基因组规模鉴定导致了 58 个推定蛋白质的表征，这些蛋白质分为 5 个亚家族，包括：12 个 ABCB、24 个 ABCC、1 个 ABCE、5 个 ABCF、15 个 ABCG，其中 51 个包含跨膜结构域。