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Complete genome sequence of a subgenotype XXI.1.1 pigeon paramyxovirus type 1 virus (PPMV‑1) isolated from Iran in 2018 and phylogenetic analysis of a possible novel, but unassigned, PPMV-1 group isolated in 2014
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2.0 ) Pub Date : 2020-10-17 , DOI: 10.1016/j.cimid.2020.101565
Reza Esmaeelzadeh Dizaji , Arash Ghalyanchilangeroudi , Mehdi Vasfi Marandi , Hossein Hosseini , Vahid Karimi , Zahra Ziafatikafi , Aidin Molouki , Mohammad Hossein Fallah Mehrabadi

Newcastle disease (ND) is one of the most serious infectious and contagious viral diseases in avian species. Recently, several ND outbreaks in pigeon caused by pigeon paramyxovirus serotype-1 (PPMV-1) have been reported from Iran, but unfortunately, phylogenetic studies have been mostly conducted on partial sequence of NDV fusion (F) gene. In addition, a complete genome data of Iranian PPMV-1 strains are not available. In the present study, a PPMV-1, named Avian avulavirus 1/pigeon/Iran/UT-EGV/2018, isolated from an infected pigeon, was subjected to whole-genome sequencing. The isolate showed an MDT of 74 h, thus categorizing it as mesogenic. The phylogenetic analysis based on the F gene sequence revealed the isolate belongs to XXI.1.1 subgenotype (min 0.9 % and max 3 %). To our knowledge, our study is the first study to publish the complete genome of a PPMV-1 from Iran. According to BLAST results, the whole genome of UT-EGV had high homology with some Russian, Egyptian and Ukrainian strains (the highest was 96.55 %). Additionally, we conducted a phylogenetic analysis on five PPMV-1 that we isolated in 2014 to find that they may belong to a completely unreported subgenotype (6 % distance when compared as a group). The information obtained from this study can be useful in preventive measures, including constructing an effective vaccine against PPMV-1 in Iran.



中文翻译:

2018年从伊朗分离的XXI.1.1亚型副粘病毒1型鸽子病毒(PPMV-1)的完整基因组序列,以及2014年分离出的可能但未分配的新型PPMV-1组的系统发育分析

新城疫(ND)是禽类中最严重的传染性和传染性病毒疾病之一。最近,伊朗报道了几只由副粘病毒血清型1(PPMV-1)引起的鸽子ND暴发,但不幸的是,系统发育研究主要是对NDV融合(F)基因的部分序列进行的。此外,还没有伊朗PPMV-1菌株的完整基因组数据。在本研究中,对从受感染的鸽子中分离出来的PPMV-1(称为禽短病毒1 /鸽子/伊朗/ UT-EGV / 2018)进行了全基因组测序。该分离物显示74 h的MDT,因此将其分类为介晶的。基于F基因序列的系统发育分析表明,该分离物属于XXI.1.1亚基因型(最小0.9%,最大3%)。据我们所知,我们的研究是第一个发表来自伊朗的PPMV-1完整基因组的研究。根据BLAST结果,UT-EGV的全基因组与一些俄罗斯,埃及和乌克兰菌株具有高度同源性(最高为96.55%)。此外,我们对2014年分离出的5种PPMV-1进行了系统发育分析,发现它们可能属于完全未报告的亚基因型(与一组相比,距离为6%)。从这项研究中获得的信息可用于预防措施,包括在伊朗构建针对PPMV-1的有效疫苗。我们对2014年分离出的5种PPMV-1进行了系统发育分析,发现它们可能属于完全未报告的亚基因型(与一组相比,距离为6%)。从这项研究中获得的信息可用于预防措施,包括在伊朗构建针对PPMV-1的有效疫苗。我们对2014年分离出的5种PPMV-1进行了系统发育分析,发现它们可能属于完全未报告的亚基因型(与一组相比,距离为6%)。从这项研究中获得的信息可用于预防措施,包括在伊朗构建针对PPMV-1的有效疫苗。

更新日期:2020-10-30
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