Konjac glucomannan (KGM) hydrolysate is a potentially valuable prebiotic that could improve gastrointestinal health by modulating the growth of probiotic bacteria and by promoting the production of short-chain fatty acids (SCFAs). In this study, we used lytic polysaccharide monooxygenases (LPMOs) to produce oligosaccharides from KGM and studied their prebiotic functions. The LPMO from Pleurotus ostreatus (PoLPMO9D) was shown to efficiently depolymerize KGM and produce a broad range of small oligomers. PoLPMO9D showed maximal activities at 50–60 °C and pH 4.0. When KGM-depolymerizing products produced by PoLPMO9D were employed as the carbon source instead of untreated KGM polymers, the growth of faecal microbiota was 2.76 times higher, a significant increase in the genus Lactococcus was observed, and the production of SCFAs increased by 14.6-fold with a significant pH decrease. This study shows that LPMOs may be a promising alternative enzyme for depolymerizing polysaccharide to prepare prebiotics from KGM.

魔芋葡甘露聚糖（KGM）水解物是潜在有价值的益生元，可通过调节益生菌的生长并促进短链脂肪酸（SCFA）的产生来改善胃肠道健康。在这项研究中，我们使用裂解多糖单加氧酶（LPMOs）从KGM生产寡糖，并研究了它们的益生元功能。来自平菇的LPMO （Po LPMO9D）被证明可以有效地解聚KGM并产生广泛的小型低聚物。Po LPMO9D在50–60°C和pH 4.0下显示最大活性。宝生产的KGM解聚产品时LPMO9D被用作碳源，而不是未经处理的KGM聚合物，粪便微生物群的生长高2.76倍，观察到乳球菌属显着增加，SCFA的产生增加了14.6倍，pH显着降低。这项研究表明，LPMOs可能是一种有前途的替代酶，用于将多糖解聚以从KGM制备益生元。