In the present work we studied the effect of 2D WS₂ nanoparticles on the conformational changes in lysozyme protein at different pH values (2.0–11.5). The contributions of various structural conformations (α-helix, β-sheets parallel and antiparallel, unordered structure and side groups) were determined by decomposition of Amid I absorbance bands. The 2D WS₂ were shown to have different impact on secondary structure depending on pH of the solution and protein concentration.

The amyloid fibril presence was confirmed with confocal microscopy enhanced by gold support, and fluorescent spectroscopy with amyloid-sensitive dye Thioflavin T. Our data show that WS₂ can both inhibit and stimulate amyloid formation. Additionally, we have also reported an unusual spectroscopic behavior displayed by lysozyme, indicated by narrowing of Amide I and Amide II bands at pH 2.5 and 3.5 when incubated with 2D WS2 nanoparticles.

在当前的工作中，我们研究了2D WS ₂纳米颗粒对不同pH值（2.0-11.5）下溶菌酶蛋白构象变化的影响。各种结构构象（α-螺旋，平行和反平行的β-折叠，无序结构和侧基）的贡献通过Amid I吸收带的分解来确定。已显示2D WS ₂对二级结构的影响不同，具体取决于溶液的pH值和蛋白质浓度。

通过金支持物增强的共聚焦显微镜和淀粉样敏感染料Thioflavin T的荧光光谱证实了淀粉样原纤维的存在。我们的数据表明WS ₂既可以抑制也可以刺激淀粉样形成。此外，我们还报告了溶菌酶显示的光谱行为异常，与2D WS2纳米粒子一起孵育时，在pH 2.5和3.5时，酰胺I和酰胺II谱带变窄。