A method for assay of rutin content in the leaves of the black poplar (Populus nigra L.) using HPLC at a wavelength of 360 nm was developed; this used an Elsico column (No. 10302, 2 mm × 80 mm; Silasorb C18 7 μm), with a mobile phase consisting of acetonitrile-water (2:8) supplemented with 1% glacial acetic acid. The suitability of the method for analysis of standard rutin samples was confirmed. Rutin content in black poplar leaves ranged from 0.67% to 1.05%. Statistical analysis of the experimental results provided evidence that the error level in single determinations of rutin in black poplar leaves with a 95% confidence interval was ± 4.6%.

中文翻译：

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黑杨叶中芦丁含量的HPLC测定

建立了一种在 360 nm 波长下使用 HPLC 测定黑杨 (Populus nigra L.) 叶片中芦丁含量的方法；这使用 Elsico 色谱柱（No. 10302，2 mm × 80 mm；Silasorb C18 7 μm），流动相由乙腈-水 (2:8) 组成，并辅以 1% 冰醋酸。确认了该方法对标准芦丁样品分析的适用性。黑杨树叶中芦丁的含量介于 0.67% 至 1.05% 之间。实验结果的统计分析表明，单次测定黑杨树叶中芦丁的误差水平为±4.6%，置信区间为 95%。