The main goal of the study was to examine and compare the inhibition effect of knotwood extractives of Scots (Pinus sylvestris) and black pine (Pinus nigra) against the two white-rot fungi and two brown-rot fungi. Knotwood was extracted in a Soxhlet apparatus. Extracts were chemically analyzed, and the inhibitory effect of purified pinosylvins and crude hydrophilic extracts was tested against growth of the white-rot fungi Schizophyllum commune and Trametes versicolor and the brown-rot fungi Fibroporia vaillantii and Gloeophyllum trabeum with the in vitro antifungal assay. Knotwood of Scots pine and black pine contained comparable amounts of total extractives. Pinosylvin, pinosylvin monomethyl ether and nortrachelogenin were characteristic compounds in knotwood extracts of both pines. Scots pine knotwood contained larger amounts of pinosylvin than black pine. The ratio between pinosylvin monomethyl ether and pinosylvin was higher in black pine knotwood. Purified pinosylvins and crude hydrophilic extracts inhibited the growth of all the white-rot and brown-rot fungi tested. Pure pinosylvins better inhibited fungal growth than crude knotwood extracts, whereas the efficiency was not unambiguous and relates to fungi species. Crude hydrophilic extracts of Scots pine more efficiently inhibited fungal growth than extracts of black pine. With the present investigation, hydrophilic extracts of pine knotwood were demonstrated as formulations of natural compounds with good antifungal properties. Broken tops of Scots pine, which can lie on forest grounds for a month or even more, still contain high amounts of phenolic extractives and are therefore a potential raw material for recovery of bioactive compounds.

中文翻译：

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苏格兰松（Pinus sylvestris）和黑松（Pinus nigra）的提取物对 Schizophyllum community、Trametes versicolor、Gloeophyllum trabeum 和 Fibroporia vaillantii 生长的体外抑制作用

该研究的主要目标是检查和比较苏格兰（Pinus sylvestris）和黑松（Pinus nigra）的虎杖提取物对两种白腐真菌和两种褐腐真菌的抑制作用。在索氏装置中提取 Knotwood。对提取物进行化学分析，并通过体外抗真菌试验测试了纯化的松香素和粗亲水提取物对白腐真菌 Schizophyllum community 和 Trametes versicolor 以及褐腐真菌 Fibroporia vaillantii 和 Gloeophyllum trabeum 的生长的抑制作用。苏格兰松和黑松的诺特木含有相当数量的总提取物。Pinosylvin、pinosylvin单甲醚和nortrachelogenin是这两种松树的虎杖提取物中的特征化合物。苏格兰松木结木比黑松含有更多的松香素。黑松木结木中松香素单甲醚和松香素的比例较高。纯化的松果苷和粗亲水提取物抑制了所有测试的白腐真菌和褐腐真菌的生长。纯松果木素比粗木结木提取物更好地抑制真菌生长，而效率不明确且与真菌种类有关。苏格兰松的粗亲水提取物比黑松提取物更有效地抑制真菌生长。在本研究中，松节材的亲水提取物被证明是具有良好抗真菌特性的天然化合物制剂。苏格兰松树的断顶，可以在森林地面上躺一个月甚至更长时间，