Iron (Fe) toxicity is one of the major abiotic stresses which limits the yield of lowland rice. This study aims to investigate the physiological, biochemical, and molecular aspects of two contrasting aromatic Joha rice, viz., Keteki and Kola Joha of Assam. Oxidative damage caused due to Fe2+ toxicity was quantitatively determined. Fe2+ toxicity in the growth medium increases the level of ROS and anti-oxidative enzyme activity. Along with the aforementioned damage caused due to Fe2+ toxicity, chlorophyll content decreases in both the rice varieties. Detection of Fe3+ and Fe2+ was also conducted by Perls' Prussian and Turnbull blue method, respectively. In addition, spectrophotometric quantification of Fe2+ was determined by 2, 2'-Bipyridyl (Bpy). Above 2.5 mM, Fe2+ toxicity was found to be lethal in rice seedlings affecting their total growth and biomass. Gene expression analysis of iron-regulated transporter 1 (OsIRT1), Yellow Stripe-Like 15 (OsYSL15), and ferritin 1 (OsFer1) revealed the differential gene expression over a time period of Fe2+ toxicity. Our study suggested that the different parameters which are considered here can be helpful for the better understanding of how aromatic Joha rice performed under Fe2+ toxicity which can also help to reveal broader aspects that how gene players are involved in the iron homeostasis mechanism in Joha rice in coming future.

中文翻译：

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印度阿萨姆邦本土芳香乔哈水稻品种铁（Fe2+）毒性反应的生理生化和分子评估

铁（Fe）毒性是限制低地水稻产量的主要非生物胁迫之一。本研究旨在调查两种截然不同的芳香乔哈大米的生理、生化和分子方面，即阿萨姆邦的 Keteki 和 Kola Joha。定量测定由 Fe2+ 毒性引起的氧化损伤。生长培养基中的 Fe2+ 毒性会增加 ROS 水平和抗氧化酶活性。除了上述由 Fe2+ 毒性引起的损害外，两个水稻品种的叶绿素含量均下降。Fe3+ 和Fe2+ 的检测也分别通过Perls 的Prussian 和Turnbull blue 方法进行。此外，Fe2+ 的分光光度定量由 2, 2'-联吡啶 (Bpy) 确定。高于 2.5 mM，研究发现，Fe2+ 毒性对水稻幼苗是致命的，会影响它们的总生长和生物量。铁调节转运蛋白 1 (OsIRT1)、黄色条纹样 15 (OsYSL15) 和铁蛋白 1 (OsFer1) 的基因表达分析揭示了 Fe2+ 毒性时间段内的差异基因表达。我们的研究表明，这里考虑的不同参数有助于更好地了解芳香乔哈大米在 Fe2+ 毒性下的表现，这也有助于揭示基因参与者如何参与乔哈大米铁稳态机制的更广泛方面。未来。