Introduction

Diabetes mellitus is a serious metabolic disorder causing multiple organ damage in human. However, the lipidomic profiles in different organs and their associations are rarely studied in either diabetic patients or animals.

Objectives

To evaluate and compare the characteristics of lipid species in serum and multiple tissues in a diabetic mouse model.

Methods

Semi-quantitative profiling analyses of intact and oxidized lipids were performed in serum and multiple tissues from a diabetic mouse model fed a high fat diet and treated with streptozotocin by using LC/HRMS and MS/MS. The total content of each lipid class, and the tissue-specific lipid species in all tissue samples were determined and compared by multivariate analyses.

Results

The diabetic mouse model displayed characteristic differences in serum and multiple organs: the brain and heart showed the largest reduction in cardiolipin, while the kidney had more alterations in triacylglycerol. Interestingly, the lipidomic differences also existed between different regions of the same organ: cardiolipin species with highly polyunsaturated fatty acyls decreased only in atrium but not in ventricle, while renal cortex showed longer fatty acyl chains for both increased and decreased triacylglycerol species than renal medulla. Importantly, diabetes caused an accumulation of lipid hydroperoxides, suggesting that oxidative stress was induced in all organs except for the brain during the development of diabetes.

Conclusions

These findings provided novel insight into the organ-specific relationship between diabetes and lipid metabolism, which might be useful for evaluating not only diabetic tissue injury but also the effectiveness of diabetic treatments.

中文翻译：

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糖尿病小鼠模型中血清和多种组织的全面脂质组分析

介绍

糖尿病是一种严重的代谢紊乱，会导致人体多器官损伤。然而，在糖尿病患者或动物中很少研究不同器官及其关联中的脂质组学概况。

目标

在糖尿病小鼠模型中评估和比较血清和多种组织中脂质种类的特征。

方法

在高脂饮食的糖尿病小鼠模型的血清和多个组织中，使用LC / HRMS和MS / MS对血清和多个组织进行了完整脂质和氧化脂质的半定量分析分析。通过多变量分析确定并比较所有脂质样品中每种脂质类别的总含量以及组织特异性脂质种类。

结果

糖尿病小鼠模型显示出血清和多个器官的特征差异：脑和心脏的心磷脂减少最大，而肾脏的三酰甘油改变更多。有趣的是，同一器官的不同区域之间也存在脂质组学差异：具有高度多不饱和脂肪酰基的心磷脂物种仅在心房中减少，而在心室中则没有，而肾皮质显示三酰基甘油物种的脂肪酰基链长于肾髓质。重要的是，糖尿病引起脂质过氧化氢的积累，这表明在糖尿病发展过程中，除脑外，所有器官均诱发了氧化应激。

结论

这些发现为了解糖尿病与脂质代谢之间的器官特异性关系提供了新颖的见解，这不仅可用于评估糖尿病组织损伤，而且可用于评估糖尿病治疗的有效性。