Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro.

细胞的行为和功能是通过多种协同工作的分子的相互作用来决定的。为了观察这些分子动力学，已经开发了追踪单一相互作用的生物物理学研究。荧光相关光谱 (FCS) 是一种光学生物物理技术，通过记录飞升尺度的信号强度来非侵入性地解析单分子。然而，使用体外测定记录这些生物分子的行为通常无法概括直接赋予动力学的体内变量的全部范围。因此，人们对观察斑马鱼斑马鱼等生物体内这些生物分子的状态越来越感兴趣。在这篇综述中，我们探讨了斑马鱼 FCS 的进展，并将这些发现与体外发现的结果进行比较和对比。