Diatraea saccharalis constitutes a threat to the sugarcane productivity, and obtaining borer tolerant cultivars is an alternative method of control. Although there are studies about the relationship between the interaction of D. saccharalis with sugarcane, little is known about the molecular and genomic basis of defense mechanisms that confer tolerance to sugarcane cultivars. Here, we analyzed the transcriptional profile of two sugarcane cultivars in response to borer attack, RB867515 and SP80-3280, which are considered tolerant and sensitive to the borer attack, respectively. A sugarcane genome and transcriptome were used for read mapping. Differentially expressed transcripts and genes were identified and termed to as DETs and DEGs, according to the sugarcane database adopted. A total of 745 DETs and 416 DEGs were identified (log₂|ratio| > 0.81; FDR corrected P value ≤ 0.01) after borer infestation. Following annotation of up- and down-regulated DETs and DEGs by similarity searches, the sugarcane cultivars demonstrated an up-regulation of jasmonic acid (JA), ethylene (ET), and defense protein genes, as well as a down-regulation of pathways involved in photosynthesis and energy metabolism. The expression analysis also highlighted that RB867515 cultivar is possibly more transcriptionally activated after 12 h from infestation than SP80-3280, which could imply in quicker responses by probably triggering more defense-related genes and mediating metabolic pathways to cope with borer attack.

糖蔗糖（diatraea saccharalis）对甘蔗生产力构成威胁，而获得耐bore虫的品种是另一种控制方法。尽管有关于糖衣藻之间相互作用关系的研究对于甘蔗，对于赋予甘蔗品种耐性的防御机制的分子和基因组基础知之甚少。在这里，我们分析了两个甘蔗品种对867虫侵袭的转录谱，分别为RB867515和SP80-3280，它们分别被认为对to虫侵袭具有耐受性和敏感性。甘蔗基因组和转录组用于读图。根据采用的甘蔗数据库，鉴定了差异表达的转录本和基因，并称为DET和DEG。总共鉴定了745个DET和416个DEG（log ₂ |比率|> 0.81； FDR校正后的P≤0.01）。在通过相似性搜索注释上调和下调的DET和DEG之后，甘蔗品种证明了茉莉酸（JA），乙烯（ET）和防御蛋白基因的上调以及途径的下调参与光合作用和能量代谢。表达分析还强调，在侵染后12 h，RB867515品种比SP80-3280的转录激活程度更高，这可能通过触发更多与防御相关的基因和介导代谢途径来应对虫蛀来暗示更快的反应。