Fungi in the family Botryosphaeriaceae are known as pathogens of diverse woody hosts, including forest tree species. Although, several of these fungi have been described in different forest hosts in Portugal, their diversity and distribution is still poorly understood. A survey was conducted across the country to identify Botryosphaeriaceae species associated with the main forest tree species in Portugal, Quercus suber, Eucalyptus globulus and Pinus pinaster. Additionally, a meta-analysis was performed to compile and organize all records known from Portugal. From this meta-analysis, 22 different Botryosphaeriaceae species were reported and 40 different plant hosts were recorded in several studies from agricultural crops to ornamental and forest species. A total of 105 host-pathogen interactions were identified. In the national survey, 12 Botryosphaeriaceae species were identified, with Diplodia corticola being the most frequent. Diplodia insularis, Diplodia pyri, Dothiorella plurivora and Dothiorella yunnana are reported for the first time in Portugal. Of the 23 different host-fungus associations identified, 10 are also recognised as new hosts. Artificial inoculation tests confirmed the pathogenicity of all species, except Dothiorella iberica, Dothiorella plurivora and Dothiorella yunnana, which are regarded as weakly or non-pathogenic to the hosts tested. Pathogenicity tests revealed the host-jump potential of some species, showing high susceptibility of Q. suber to Neofusicoccum parvum and N. eucalyptorum and of P. pinaster to D. corticola. Our results show that Botryosphaeriaceae species have a widespread distribution across the country, however some species such as D. corticola, D. sapinea and N. eucalyptorum exhibit a distribution that overlaps the distribution of their preferred hosts.

葡萄球菌科中的真菌被称为多种木本宿主的病原体，包括森林树种。尽管在葡萄牙的不同森林寄主中已经描述了其中几种真菌，但对其多样性和分布仍然知之甚少。在全国范围内进行了一项调查，以确定与葡萄牙主要林木树种相关的葡萄孢菌种、,栎，桉树和松树。此外，进行了荟萃分析，以汇编和整理葡萄牙已知的所有记录。通过这项荟萃分析，报告了22种不同的葡萄球菌物种，并在从农作物到观赏植物和森林物种的数项研究中记录了40种不同的植物宿主。总共鉴定出105种宿主-病原体相互作用。在国家调查中，鉴定出12种葡萄球菌，最常见的是Diplodia corticola。双翅目Diplodia insularis，双翅目Diplodia pyri，百日咳Dothiorella plurivora和云南Dothiorella yunnana在葡萄牙首次报道。在确定的23个不同的宿主-真菌协会中，有10个也被视为新的宿主。人工接种试验证实了除金毛杜鹃，多毛杜氏藻和云南杜氏菌之外的所有物种的致病性，它们对所测试的宿主均具有弱或非致病性。致病性试验表明一些物种的主机跳电势，表现出的高敏感性Q.苏伯到Neofusicoccum孢子虫和N. eucalyptorum和P.松树至D. corticola。我们的结果表明，葡萄球菌属物种在全国范围内分布广泛，但是某些物种如D. corticola，D。sapinea和N. eucalyptorum的分布与其首选寄主的分布重叠。