Cardiotoxicity from long-term use of the anticancer drug, doxorubicin (DOX), imposes a strict limitation its administration. The health benefits of avocado seeds (AS) stem from essential nutrients and phytochemicals. This study aimed to compare effects of ethanol (EAS), and water (WAS) extracts from AS on DOX-induced cardiotoxicity. Forty adult male albino rats were divided into four groups: GI were control rats. GII rats received DOX alone over a 2 week period. GIII animals received the DOX treatment plus AS. GIV rats received the DOX treatment plus EAS. AS extract treatments were administered after initiation of cardiotoxicity. Total flavonoids, polyphenols, and antioxidant capacity of both AS extracts were measured. Serum markers of cardiac dysfunction were evaluated, oxidative stress and inflammatory biomarkers were measured in cardiac tissue homogenates and histopathology of heart tissues was evaluated. The AS ethanolic extract had a higher content of active compounds. DOX treatment increased markers of cardiac dysfunction Matrix Metalloproteinase-1 (MMP-1), Serum Cardiac Troponin I (cTnI), Lactate Dehydrogenase (LDH), Serum Creatine Kinase Isoenzyme-MB (CK-MB), and Heart-Type Fatty Acid-Binding Protein (FABP3) and produced increases in levels of Advanced Oxidation Protein Products (AOPP) and Malondialdehyde (MDA) in cardiac tissue homogenates with a significant reduction in Catalase (CAT), Total Antioxidant Capacity (TAC). It also increased levels of Tumor Necrosis Factor (TNF-a), Interleukin-6 (IL-6), Interleukin-1 beta (IL-1ß) and Caspase-3, in the cardiac tissue homogenates. EAS was more effective than WAS, but both acted as cardioprotective agents following DOX treatment as evidenced by mitigation of impacts on biochemical markers.

长期使用抗癌药阿霉素（DOX）会产生心脏毒性，因此其使用受到严格限制。鳄梨种子（AS）的健康益处来自必需的营养物质和植物化学物质。这项研究旨在比较AS中乙醇（EAS）和水（WAS）提取物对DOX诱导的心脏毒性的影响。将40只成年雄性白化病大鼠分为四组：胃肠道为对照组。GII大鼠在2周内仅接受DOX。GIII动物接受DOX加AS治疗。GIV大鼠接受DOX治疗加EAS。在心脏毒性开始后进行AS提取物治疗。测定了两种AS提取物的总类黄酮，多酚和抗氧化能力。评估了心脏功能障碍的血清标志物，测量心脏组织匀浆中的氧化应激和炎性生物标志物，并评估心脏组织的组织病理学。AS乙醇提取物具有较高的活性化合物含量。DOX治疗可增加心脏功能障碍基质金属蛋白酶-1（MMP-1），血清心肌肌钙蛋白I（cTnI），乳酸脱氢酶（LDH），血清肌酸激酶同工酶-MB（CK-MB）和心脏型脂肪酸-结合蛋白（FABP3）在心脏组织匀浆中产生的高级氧化蛋白产物（AOPP）和丙二醛（MDA）含量增加，过氧化氢酶（CAT），总抗氧化能力（TAC）明显降低。它也增加了心脏组织匀浆中的肿瘤坏死因子（TNF-a），白细胞介素6（IL-6），白细胞介素1 beta（IL-1ß）和Caspase-3的水平。