Whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is one of the most harmful pests in greenhouses and in open fields. The present study aimed to assess in vitro virulence of 3 entomopathogenic fungal strains (EPFs) of Lecanicillium lecanii 3 (V-3), 4 (V-4), and 5 (V-5) against the whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), by spray method. The 3 disparate bioassays were performed encompassed of conidial concentrations and fungal filtrate of the strains, V-3, V-4, and V-5, and also their binary combinations. In the filtrate bioassay, 2 ml of fungal filtrate of each strain was used. In the conidial bioassay, 3 disparate concentrations (1 × 105, 1 × 106, and 1 × 107 conidia ml−1) were used for each fungal strain, while in the binary combinations (1 ml filtrate + 1 ml conidia) of V-3 × V-3, V-4 × V-4, and V-5 × V-5 were used. Mortality rates against the whitefly were recorded on the 7th day. In the conidial bioassay, maximum mortality rates were found at V-3 strain (90.6%), V-4 strain (78.4%), and V-5 strain (83.6%) at the highest concentration (1 × 107 conidia ml−1) on the 7th day. In the filtrate bioassay, V-3 strain revealed a maximum mortality (93%), V-4 strain (85%), and then V-5 strain (87%) on the 7th day. Moreover, in the bioassay of binary combinations, the highest mortality rate of the whitefly was counted in V-3 × V-3 strain (84.6%), V-4 × V-4 strain (70.6%), and V-5 × V-5 strain (79.8%) on the 7th day. All treatments had the potential to control B. tabaci significantly. In all bioassays, the V-3 strain was the extreme virulent, and the filtrate application of V-3 strain was the utmost impressive against B. tabaci.

中文翻译：

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三种Lecanicillium lecanii菌株对粉虱Bemisia tabaci（Genn。）的体外毒力（半翅目：鞘翅目）

烟粉虱，烟粉虱（Bemisia tabaci，Genn。）（半翅目：Aleyrodidae），是温室和露天场所中最有害的害虫之一。本研究旨在评估Lecanicillium lecanii 3（V-3），4（V-4）和5（V-5）的3种昆虫病原真菌菌株（EPF）对粉虱Bemisia tabaci（Genn。 ）（半翅目：A科），通过喷雾法。进行了3种不同的生物测定，包括菌株V-3，V-4和V-5的分生孢子浓度和真菌滤液，以及它们的二元组合。在滤液生物测定中，使用每种菌株2ml的真菌滤液。在分生孢子生物测定中，每个真菌菌株使用3种不同浓度（1×105、1×106和1×107分生孢子ml-1），而在二元组合（1 ml滤液+ 1 ml分生孢子）中使用V-使用了3×V-3，V-4×V-4和V-5×V-5。在第7天记录针对粉虱的死亡率。在分生孢子生物测定法中，最高浓度（1×107分生孢子ml-1）的V-3株（90.6％），V-4株（78.4％）和V-5株（83.6％）发现最高死亡率。 ）在第7天。在滤液生物测定中，在第7天，V-3株显示最高死亡率（93％），V-4株显示（85％），然后是V-5株（87％）。此外，在二元组合的生物测定中，粉虱的最高死亡率在V-3×V-3菌株（84.6％），V-4×V-4菌株（70.6％）和V-5×第7天的V-5株（79.8％）。所有处理均具有显着控制烟粉虱的潜力。在所有生物测定中，V-3菌株都具有极强的毒性，而V-3菌株的滤液应用对烟粉虱的影响最大。