A Study on Serological Reactivity Profile of Different Antigen Preparations with Bancroftian filariasis Human Infection Sera,Protein & Peptide Letters

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A Study on Serological Reactivity Profile of Different Antigen Preparations with Bancroftian filariasis Human Infection Sera
Protein & Peptide Letters ( IF 1.0 ) Pub Date : 2020-08-31 , DOI: 10.2174/0929866527666200225123534
Mohd Saeed ₁ , Vikas Kushvaha ₂ , Syed Mohd Faisal ₃ , Richa Verma ₄ , Irfan Ahmad ₄ , Huma Mustafa ₅ , Magdah Ganash ₆ , Mohammad Amjad Kamal ₇ , Ghulam Md Ashraf ₇

Affiliation

Background: Lymphatic Filariasis (LF) is one of the incapacitating and mosquito-borne sicknesses that on progression may prompt a few recognizable types of clutters like extreme lymphedema, hydrocele, and elephantiasis.

Methods: Antigenic preparations of B. malayi adult (BmA), S. cervi adult parasites and microfilariae (mf) total parasite extract were used to analyze the serological reactivity profile with human infectious sera collected from endemic areas of Bancroftian filariasis by performing Western blot and ELISA analysis. Sera from healthy human subjects were also included in the study to determine the variation incurred in the reactivity due to the filariasis infection. Gelelectrophoresis analysis of the crude-extract of BmA revealed seven protein bands while more than ten bands were recognized in S. cervi.

中文翻译：

班氏丝虫病人感染血清不同抗原制剂的血清反应性研究

背景：淋巴丝虫病（LF）是一种无行为能力且由蚊子传播的疾病，进展时可能会引起一些可识别的杂波类型，例如极端淋巴水肿，鞘膜积液和象皮病。

方法：使用B. malayi成虫（BmA），S。cervi成虫和微丝aria虫（mf）总寄生虫提取物的抗原制剂，通过免疫印迹和Bancroftian丝虫病流行地区收集的人感染血清分析其血清反应性。 ELISA分析。来自健康人类受试者的血清也包括在该研究中，以确定由丝虫病感染引起的反应性变化。BmA粗提物的凝胶电泳分析揭示了七个蛋白带，而在酿酒酵母中则识别出十个以上的带。

更新日期：2020-10-16

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