Rapid identification of methicillin-sensitive Staphylococcus aureus (MSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-intermediate S. aureus (VISA) is important for accurate treatment, timely intervention, and prevention of outbreaks. Here, 90 S. aureus isolates were analyzed for protein biomarker discovery, including MSSA, vancomycin-susceptible S. aureus (VSSA), hVISA, and VISA strains. Label-free data-independent acquisition proteomics was used to identify protein biomarkers that allow for discrimination among MSSA, hVISA, and VISA strains. There were 8786 nonredundant peptides identified, corresponding to 418 different annotated nonredundant proteins. Two VISA protein biomarkers, two hVISA protein biomarkers, and one MSSA protein biomarker with high sensitivities and specificities were discovered and verified. Data are available via MassIVE with identifier MSV000085776.

中文翻译：

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使用无标记的独立于数据的获取蛋白质组学对甲氧西林敏感，异种万古霉素中间和万古霉素中间金黄色葡萄球菌菌株的蛋白质生物标记物发现

快速鉴定对甲氧西林敏感的金黄色葡萄球菌（MSSA），异源万古霉素中间金黄色葡萄球菌（hVISA）和万古霉素中间金黄色葡萄球菌（VISA）对于准确治疗，及时干预和预防暴发至关重要。在这里，分析了90种金黄色葡萄球菌分离物的蛋白质生物标志物发现，包括MSSA，对万古霉素敏感的金黄色葡萄球菌（VSSA），hVISA和VISA菌株。使用无标签数据独立的获取蛋白质组学来鉴定可区分MSSA，hVISA和VISA菌株的蛋白质生物标记。鉴定出了8786个非冗余肽，对应于418种不同的带注释的非冗余蛋白。发现并验证了两种VISA蛋白生物标记物，两种hVISA蛋白生物标记物和一种MSSA蛋白生物标记物，它们具有很高的敏感性和特异性。可以通过MassIVE使用标识符为MSV000085776的数据。