The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2.

中文翻译：

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超声微泡介导的 microRNA-505 通过 AKT2 调节宫颈癌细胞生长

超声和微泡 (USMB-) 介导的 microRNA (miR) 的应用是一种很有前景的基因递送方法，用于癌症治疗。我们旨在讨论 USMB-miR-505 对宫颈癌 (CC) 发展的影响。制备了 USMB 介导的 miR-505。研究了miR-505对其转染效率的影响以及miR-505对HeLa细胞增殖、细胞周期、凋亡、迁移和侵袭的影响。预测miR-505的靶基因，并检测其在CC中的表达。进一步验证了靶基因对HeLa细胞的作用。USMB-miR-505 显示出比单独的 miR-505 更高的转染效率。USMB介导的miR-505对HeLa细胞的抑制作用优于miR-505。miR-505 靶向 AKT2，后者在 CC 中上调。AKT2的过表达逆转了USMB-miR-505对HeLa细胞恶性行为的抑制作用。总体而言，我们强调 USMB-miR-505 通过靶向 AKT2 抑制 HeLa 细胞恶性行为。