C-dots synthesized from carbon nanopowder (oxidation, hydrothermal) are particularly attractive theragnostic agents for bone-related injuries and disease due to their bright fluorescence and high binding affinity and specificity for bones, as demonstrated in a larval animal model. Larval bone development, however, is significantly different from the bone growth, repair and regeneration processes occurring in adults. Using adult zebrafish, we investigated C-dots interactions with adult skeletal structures. Upon injection, C-dots were observed at the surface of bones, at sites of appositional growth. In regenerating bones, C-dots were observed at the core and on the surface of the bones depending on the age of the tissue. C-dots deposition occurred within 30 min of delivery and it was highly selective. Importantly, their deposition did not interfere with bone regeneration or the animals health. Together, these properties establish C-dots as novel tools for the diagnostic and treatment of adult bone-related injuries and diseases.

中文翻译：

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成年骨骼中的碳点沉积揭示了生长，损伤和再生的区域

如幼虫动物模型所示，由于碳纳米粉（氧化，水热法）合成的C-点因其明亮的荧光以及对骨骼的高结合亲和力和特异性而特别有吸引力，因此可用于治疗与骨相关的损伤和疾病。但是，幼虫的骨骼发育与成年人的骨骼生长，修复和再生过程明显不同。使用成年斑马鱼，我们调查了成年骨骼结构的C点相互作用。注射后，在骨骼表面，并位生长部位观察到C点。在再生骨骼中，根据组织的年龄，在骨骼的核心和表面会观察到C点。碳点沉积在交货后30分钟内发生，并且具有很高的选择性。重要的，它们的沉积不会影响骨骼再生或动物健康。这些特性一起将C点确立为诊断和治疗成人骨骼相关伤害和疾病的新颖工具。