Trehalose plays a crucial role in the diapause process of many insects, serving as an energy source and a stress protectant. Trehalose accumulation has been reported in diapause pupae of Antheraea pernyi; however, trehalose metabolic regulatory mechanisms associated with diapause termination remain unclear. Here, we showed that the enhanced trehalose catabolism was associated with an increase in endogenous 20-hydroxyecdysone (20E) in hemolymph of A. pernyi pupae during their diapause termination and posttermination period. Injection of 20E increased the mRNA level of trehalase 1A (ApTre-1A) and trehalase 2 (ApTre-2) of A. pernyi diapause pupae in a dose-dependent manner but did not affect the mRNA level of trehalase 1B (ApTre-1B). Meanwhile, exogenous 20E increased the enzyme activities of soluble and membrane-bound trehalase, leading to a decline in hemolymph trehalose. Conversely, the expression of ApTre-1A and ApTre-2 were down-regulated after the ecdysone receptor gene (ApEcRB1) was silenced by RNA interference or by injection of an ecdysone receptor antagonist cucurbitacin B (CucB), which inhibits the 20E pathway. Moreover, CucB treatment delayed adult emergence, which suggests that ApEcRB1 might be involved in regulating pupal-adult development of A. pernyi by mediating ApTre-1A and ApTre-2 expressions. This study provides an overview of the changes in the expression and activity of different trehalase enzymes in A. pernyi in response to 20E, confirming the important role of 20E in controlling trehalose catabolism during A. pernyi diapause termination and posttermination period.

中文翻译：

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海藻糖代谢在调制的20-羟基柞蚕（鳞翅目：蚕蛾）在其滞育解除和终止后时代

海藻糖在许多昆虫的滞育过程中起着至关重要的作用，是一种能源和压力保护剂。在百花per的滞育p中有海藻糖的积累; 然而，与滞育终止有关的海藻糖代谢调节机制仍不清楚。在这里，我们表明，增强海藻糖分解代谢物的增加在血淋巴中的内源性20-羟基（20E）相关联柞蚕其滞育终止和posttermination期间蛹。注射20E会增加多年曲霉的海藻糖酶1A（ApTre-1A）和海藻糖酶2（ApTre-2）的mRNA水平。滞育p，但不影响海藻糖酶1B（ApTre-1B）的mRNA水平。同时，外源20E增加了可溶性和膜结合海藻糖酶的酶活性，导致血淋巴海藻糖的减少。相反，在蜕皮激素受体基因（ApEcRB1）通过RNA干扰或注射蜕皮激素受体拮抗剂葫芦素B（CucB）使20E通路沉默后，ApTre-1A和ApTre-2的表达下调。此外，CucB治疗延迟了成年人的出现，这表明ApEcRB1可能通过介导ApTre-1A参与调节pernyi的p成虫发育。和ApTre-2表达式。本研究提供了在不同的海藻糖酶的表达和酶活性的变化的概述柞蚕响应于20E，20E确认在期间控制海藻糖分解代谢中的重要作用柞蚕滞育终止和posttermination周期。