The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the most destructive pests to cruciferous plants worldwide. The oligophagous moth primarily utilizes its host volatiles for foraging and oviposition. Chemosensory proteins (CSPs) are soluble carrier proteins with low molecular weight, which recognize and transport various semiochemicals in insect chemoreception. At present, there is limited information on the recognition of host volatiles by CSPs of P. xylostella. Here, we investigated expression patterns and binding characteristics of PxylCSP11 in P. xylostella. The open reading frame of PxylCSP11 was 369-bp encoding 122 amino acids. PxylCSP11 possessed four conserved cysteines, which was consistent with the typical characteristic of CSPs. PxylCSP11 was highly expressed in antennae, and the expression level of PxylCSP11 in male antennae was higher than that in female antennae. Fluorescence competitive binding assays showed that PxylCSP11 had strong binding abilities to several ligands, including volatiles of cruciferous plants, and (Z)-11-hexadecenyl acetate (Z11-16:Ac), a major sex pheromone of P. xylostella. Our results suggest that PxylCSP11 may play an important role in host recognition and spouse location in P. xylostella.

中文翻译：

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小菜蛾小菜蛾化学感测蛋白PxylCSP11的表达谱和结合特性（鳞翅目：Plutellidae）

小菜蛾，菜蛾属（鳞翅目菜蛾科）是最具有破坏性的害虫在全球范围内十字花科植物之一。少食蛾主要利用其宿主挥发物进行觅食和产卵。化学感觉蛋白（CSP）是低分子量的可溶性载体蛋白，可识别和转运昆虫化学感受态中的各种化学信息素。目前，关于小菜蛾的CSP识别宿主挥发物的信息有限。在这里，我们调查的表达模式，并结合特性Pxyl在CSP11小菜蛾。Pxyl CSP11的开放阅读框为369-bp，编码122个氨基酸。苯甲酰CSP11具有四个保守的半胱氨酸，这与CSP的典型特征是一致的。PxylCSP11在触角中高表达，PxylCSP11在雄性触角中的表达水平高于雌性触角。荧光竞争结合试验表明，Pxyl CSP11对几种配体具有很强的结合能力，其中包括十字花科植物的挥发物，以及（Z）-11-十六碳烯基乙酸酯（Z11-16：Ac），这是小菜蛾的主要性信息素。我们的结果表明Pxyl CSP11可能在X.ostostella宿主识别和配偶定位中起重要作用。