The therapeutic use of medical Cannabis is growing, and so is the need for standardized and therapeutically stable Cannabis products for patients. The therapeutic effects of Cannabis largely depend on the content of its pharmacologically active secondary metabolites and their interactions, mainly terpenoids and phytocannabinoids. Once harvested and during storage, these natural compounds may decarboxylate, oxidize, isomerize, react photochemically, evaporate and more. Despite its widespread and increasing use, however, data on the stability of most of the plant’s terpenoids and phytocannabinoids during storage is scarce. In this study, we therefore aimed to determine postharvest optimal storage conditions for preserving the composition of naturally biosynthesized secondary metabolites in Cannabis inflorescences and Cannabis extracts. To this end, Cannabis inflorescences (whole versus ground samples) and Cannabis extracts (dissolved in different solvents) from (-)-Δ⁹-trans-tetrahydrocannabinol- or cannabidiol-rich chemovars, were stored in the dark at various temperatures (25, 4, −30 and −80°C), and their phytocannabinoid and terpenoid profiles were analyzed over the course of 1 year. We found that in both Cannabis inflorescences and extracts, a storage temperature of 25°C led to the largest changes in the concentrations of the natural phytocannabinoids over time, making this the most unfavorable temperature compared with all others examined here. Olive oil was found to be the best vehicle for preserving the natural phytocannabinoid composition of the extracts. Terpenoid concentrations were found to decrease rapidly under all storage conditions, but temperatures lower than −20°C and grinding of the inflorescences were the least favorable conditions. Overall, our conclusions point that storage of whole inflorescences and extracts dissolved in olive oil, at 4°C, were the optimal postharvest conditions for Cannabis.

医疗的治疗用途 大麻 越来越多，因此对标准化和治疗稳定的需求也在增长 大麻面向患者的产品。的治疗作用大麻在很大程度上取决于其药理活性次生代谢物的含量及其相互作用，主要是萜类和植物大麻素。这些天然化合物一旦被收获并在储存过程中，可能会脱羧，氧化，异构化，发生光化学反应，蒸发等。尽管其广泛使用和不断增加的用途，但是关于大多数植物萜类和植物大麻素在储存过程中稳定性的数据很少。因此，在这项研究中，我们旨在确定收获后最佳的保存条件，以保存天然生物合成的次生代谢产物的组成。大麻 花序和 大麻提取物。为此，大麻 花序（整个与地面样本）和 大麻提取物（溶解在不同的溶剂中）（ - ） - Δ ⁹ -反式将富含四氢大麻酚或大麻二酚的化学试剂在不同温度（25、4，-30和-80°C）下避光保存，并在1年的时间里分析了它们的植物大麻素和萜类化合物谱。我们发现大麻花序和提取物的储存温度为25°C，导致天然植物大麻素的浓度随时间变化最大，与本文中所有其他检测相比，这是最不利的温度。发现橄榄油是保存提取物中天然植物大麻素成分的最佳载体。萜类化合物的浓度在所有存储条件下均会迅速降低，但温度低于-20°C，而花序的研磨是最不利的条件。总体而言，我们的结论指出，将整个花序和溶于橄榄油的提取物在4°C下储存是最佳的收获后条件。大麻。