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Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey
Journal of Veterinary Research ( IF 1.3 ) Pub Date : 2020-10-15 , DOI: 10.2478/jvetres-2020-0066
Mehmet Cemal Adiguzel 1 , Mehmet Ozkan Timurkan 2 , Seyda Cengiz 1
Affiliation  

Abstract Introduction Avian polyomavirus (APV) and psittacine beak and feather disease virus (PBFDV) induce contagious and persistent diseases that affect the beaks, feathers, and immune systems of companion birds. APV causes hepatitis, ascites, hydropericardium, depression, feather disorders, abdominal distension, and potentially death. PBFDV can induce progressive beak deformity, feather dystrophy, and plumage loss. We conducted the first prevalence survey of both APV and PBFDV infections in companion birds in eastern Turkey. Material and Methods A total of 113 fresh dropping samples from apparently healthy companion birds were collected in a random selection. The dropping samples were analysed for PBFDV and APV by PCR. Positive samples were sequenced with the Sanger method. The sequence was confirmed through alignment and the phylogenetic tree generated through the maximum likelihood method computationally. Results PBFDV and APV were detected in a respective 48.7% and 23.0% of samples. Coinfection was found in 12.4% of the samples, these all being from budgerigars (Melopsittacus undulatus). APV and PBFDV were detected in budgerigar and cockatiel (Nymphicus hollandicus) samples. Conclusion This report provides a foundation for future studies on the influence of these viruses on the health of companion birds. These high positive rates for both pathogens emphasise that healthy M. undulatus and N. hollandicus in eastern Turkey may be prone to the emergence and spread of APV and PBFDV with subclinical potential.

中文翻译:

土耳其东部伴鸟禽多瘤病毒和鹦鹉喙羽病病毒调查及序列分析

摘要 简介 禽多瘤病毒 (APV) 和鹦鹉喙羽病病毒 (PBFDV) 会诱发传染性和持续性疾病,这些疾病会影响伴侣鸟类的喙、羽毛和免疫系统。APV 会导致肝炎、腹水、心包积水、抑郁、羽毛异常、腹胀和潜在的死亡。PBFDV 可导致进行性喙部畸形、羽毛营养不良和羽毛脱落。我们对土耳其东部伴鸟中的 APV 和 PBFDV 感染进行了首次流行率调查。材料与方法 随机抽取了 113 份来自看似健康的伴侣鸟的新鲜粪便样本。通过 PCR 分析滴样的 PBFDV 和 APV。阳性样品采用 Sanger 方法测序。通过比对确认序列,并通过计算最大似然法生成系统发育树。结果 PBFDV 和 APV 分别在 48.7% 和 23.0% 的样本中检测到。在 12.4% 的样本中发现了共感染,这些样本都来自虎皮鹦鹉(Melopsittacus undulatus)。在虎皮鹦鹉和玄凤鹦鹉 (Nymphicus hollandicus) 样本中检测到 APV 和 PBFDV。结论 本报告为未来研究这些病毒对伴侣鸟健康的影响奠定了基础。这两种病原体的高阳性率强调了土耳其东部健康的 M. undulatus 和 N. hollandicus 可能容易出现具有亚临床潜力的 APV 和 PBFDV 的出现和传播。结果 PBFDV 和 APV 分别在 48.7% 和 23.0% 的样本中检测到。在 12.4% 的样本中发现了共感染,这些样本都来自虎皮鹦鹉(Melopsittacus undulatus)。在虎皮鹦鹉和玄凤鹦鹉 (Nymphicus hollandicus) 样本中检测到 APV 和 PBFDV。结论 本报告为未来研究这些病毒对伴侣鸟健康的影响奠定了基础。这两种病原体的高阳性率强调了土耳其东部健康的 M. undulatus 和 N. hollandicus 可能容易出现具有亚临床潜力的 APV 和 PBFDV 的出现和传播。结果 PBFDV 和 APV 分别在 48.7% 和 23.0% 的样本中检测到。在 12.4% 的样本中发现了共感染,这些样本都来自虎皮鹦鹉(Melopsittacus undulatus)。在虎皮鹦鹉和玄凤鹦鹉 (Nymphicus hollandicus) 样本中检测到 APV 和 PBFDV。结论 本报告为未来研究这些病毒对伴侣鸟健康的影响奠定了基础。这两种病原体的高阳性率强调了土耳其东部健康的 M. undulatus 和 N. hollandicus 可能容易出现具有亚临床潜力的 APV 和 PBFDV 的出现和传播。结论 本报告为未来研究这些病毒对伴侣鸟健康的影响奠定了基础。这两种病原体的高阳性率强调了土耳其东部健康的 M. undulatus 和 N. hollandicus 可能容易出现具有亚临床潜力的 APV 和 PBFDV 的出现和传播。结论 本报告为未来研究这些病毒对伴侣鸟健康的影响奠定了基础。这两种病原体的高阳性率强调了土耳其东部健康的 M. undulatus 和 N. hollandicus 可能容易出现具有亚临床潜力的 APV 和 PBFDV 的出现和传播。
更新日期:2020-10-15
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