This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

中文翻译：

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公猪精液冷冻保存的新精子选择标准

这项研究旨在确定潜在的标志物，可以确定射精是否适合冷冻保存。将11头公猪的新鲜精液（4-7枚射精/公猪），不管它们的精子活力如何，都要进行冷冻保存。根据精子膜的通透性和顶体完整性评估冷冻前后的精子质量。结果表明，有可能有效地冷冻射精，使其低于新鲜运动精子的70–80％的公认标准，并且仍然可以获得较高的冷冻存活率。此外，发现具有凋亡样变化的活精子的百分比，具有反应的顶体的活精子的百分比与冷冻存活率之间存在显着相关性。