Abstract

Hyaluronic acid (HA) eye drop solution is widely used to treat and manage various corneal diseases like keratoconus (after corneal cross-linking) and dry eye syndrome. However, ocular dosage forms like eye drop solution affect the routine life style of patients due to frequent dosing schedule. In this study, HA and reduced graphene oxide (rGO) was directly loaded in the silicon contact lenses (HA-GO-DL) and compared with the conventional soaking method (HA-GO-SM). The contact lenses at lower level of rGO showed permissible swelling and transmittance properties. The water retention property of HA-GO-DL contact lenses was confirmed by water evaporation studies. The flux data of HA-GO-SM contact lenses showed high burst release with 24 h release duration. While, HA-GO-DL lenses confirmed low burst with sustained release up to 96 h. In ocular irritation study, the HA-GO-DL-2 lenses was found to be safe. The HA-GO-DL-2 batch showed high HA-tear fluid concentration (rabbit model) and improvement in the rabbit tear fluid volume (Schirmer strip studies) in comparison to the soaking method (HA-GO-SM-2) and eye drop solution. The study successfully demonstrate the potential of HA-GO loaded contact lenses to improve tear fluid volume to manage various ocular diseases like dry eye syndrome.

摘要

透明质酸 (HA) 滴眼液广泛用于治疗和管理各种角膜疾病，如圆锥角膜（角膜交联后）和干眼症。然而，眼药水等眼部剂型由于频繁的给药计划而影响患者的日常生活方式。在这项研究中，HA 和还原氧化石墨烯 (rGO) 直接加载到硅隐形眼镜 (HA-GO-DL) 中，并与传统浸泡方法 (HA-GO-SM) 进行比较。rGO 含量较低的隐形眼镜表现出允许的溶胀和透射特性。HA-GO-DL 隐形眼镜的保水性通过水分蒸发研究得到证实。HA-GO-SM 隐形眼镜的通量数据显示高爆发性释放，释放持续时间为 24 小时。同时，HA-GO-DL 镜片证实了低爆破，持续释放长达 96 小时。在眼部刺激研究中，HA-GO-DL-2 镜片被发现是安全的。与浸泡方法 (HA-GO-SM-2) 和眼睛相比，HA-GO-DL-2 批次显示出高 HA-泪液浓度（兔模型）和兔泪液体积的改善（Schirmer 条研究）滴溶液。该研究成功证明了装载 HA-GO 的隐形眼镜在改善泪液量以管理各种眼部疾病（如干眼症）方面的潜力。