ABSTRACT

Nesfatin-1 and neuronostatin in the central nervous system participate in regulating stress responses. Glucocorticoid hormones affect the brain through glucocorticoid receptors (GR). We investigated in the rat the possibility of co-localizing nesfatin-1 and neuronostatin neurons in hypothalamic areas with GR. using immunohistochemistry. We counted nesfatin-1 and neuronostatin stained neurons. We counted GR positive nesfatin-1 neurons in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) and GR positive neuronostatin neurons in the periventricular nucleus (PeN). The percentage of nesfatin-1 neurons that expressed GR was 38.4% in female rats and 21.9% in male rats in the ARC, and 33.3% in female rats and 29.2% in male rats in the PVN. The percentage of neuronostatin neurons that expressed GR was 39.1% in female rats and 39.9% in male rats in the PeN. We found that a substantial portion of nesfatin-1 and neuronostatin neurons were stained for GR. We speculate that the pattern of GR might permit secretion of neuropeptides to be stimulated by peripheral glucocorticoid signals. Stress can suppress food intake, in part, through the GR in neurons that express nesfatin-1, which is a satiety molecule, and in neurons that express neuronostatin, which is an anorexigenic peptide.

摘要

中枢神经系统中的 Nesfatin-1 和neuronostatin 参与调节应激反应。糖皮质激素通过糖皮质激素受体 (GR) 影响大脑。我们在大鼠中研究了将 nesfatin-1 和神经抑制素神经元与 GR 共定位在下丘脑区域的可能性。使用免疫组织化学。我们计算了nesfatin-1 和neuronostatin 染色的神经元。我们计数了弓状核 (ARC) 和室旁核 (PVN) 中的 GR 阳性 nesfatin-1 神经元和脑室周围核 (PeN) 中的 GR 阳性神经抑制素神经元。ARC中表达GR的nesfatin-1神经元百分比在雌性大鼠中为38.4%，在雄性大鼠中为21.9%，在PVN中雌性大鼠中为33.3%，雄性大鼠中为29.2%。表达 GR 的神经抑制素神经元的百分比在雌性大鼠和 39. 9% 在 PenN 的雄性大鼠中。我们发现很大一部分 nesfatin-1 和neuronostatin 神经元被 GR 染色。我们推测 GR 的模式可能允许神经肽的分泌受到外周糖皮质激素信号的刺激。压力可以部分地通过表达nesfatin-1（一种饱腹感分子）的神经元和表达neuronostatin（一种厌食肽）的神经元中的GR来抑制食物摄入。