Objective: to determine whether skeletal muscle molecular markers and SC number were influenced differently in users and non-users of oral contraceptives (OCs) following 10 weeks of resistance training Methods: Thirty-eight young healthy untrained users (n=20) and non-users of OC (n=18) completed a 10-week supervised progressive resistance training program. Before and after the intervention a muscle tissue sample was obtained from the vastus lateralis muscle for analysis of muscle fiber cross sectional area (fCSA), and satellite cell (SC) and myonuclei number using immunohistochemistry, gene expression using PCR, protein expression and myosin heavy chain composition. Results: Following the training period quadriceps fCSA (p<0.05), SCs/ type I fiber (p=0.05) and MURF-1 mRNA (p<0.01) were significantly increased with no difference between the groups. However, SCs/total fiber and SCs/type II fiber increased in OC users only, and SCs/ type II fCSA tended (p=0.055) to be greater in the OC-users. Furthermore, in OC users there were a fiber type shift from MHC IIx to MHC IIa (p<0.01) and expression of MRF4 mRNA (p<0.001) was significantly greater than in non-OC users. Conclusion: Use of 2^nd generation OCs in young untrained women increased skeletal muscle MRF4 expression and SC number following 10 weeks of resistance training compared to non-users.

中文翻译：

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口服避孕药使用者和非使用者进行抗性训练10周后骨骼肌肥大的分子标记

目的：确定口服避孕药（OCs）使用者和非使用者在接受10周抗性训练后骨骼肌分子标志物和SC数量是否受到不同的影响方法：38名年轻健康未经训练的使用者（n = 20） OC（n = 18）的用户完成了一个为期10周的有监督的渐进式阻力训练计划。在干预前后，从外侧股外侧肌中获取肌肉组织样品，用于使用免疫组织化学，使用PCR的基因表达，蛋白表达和肌球蛋白重链分析肌纤维横截面积（fCSA），卫星细胞（SC）和肌核数。链组成。结果：训练结束后，股四头肌fCSA（p <0.05），SCs / I型纤维（p = 0.05）和MURF-1 mRNA（p <0）。01）明显增加，两组之间无差异。但是，仅在OC用户中，SCs /总光纤和SCs / II型光纤增加，并且在OC用户中，SCs / II型fCSA趋于更大（p = 0.055）。此外，在OC用户中，纤维类型从MHC IIx变为MHC IIa（p <0.01），MRF4 mRNA的表达（p <0.001）明显大于非OC用户。结论：使用2^ND在年轻妇女未受过训练的一代法团以下10周阻力训练的相对于非用户增加骨骼肌MRF4表达和SC号码。