Isolation and Identification of Streptomyces sp. Act4Zk, a Good Producer of Staurosporine and Some Derivatives,Letters in Applied Microbiology

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Isolation and Identification of Streptomyces sp. Act4Zk, a Good Producer of Staurosporine and Some Derivatives
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-10 , DOI: 10.1111/lam.13415
Z. Khosravi Babadi _{1,

2} , G. Ebrahimipour ₁ , J. Wink ₂ , A. Narmani _{3,

4} , C. Risdian _{2,

5}

Affiliation

In the present study, strain Streptomyces sp. Act4Zk was isolated based on a method developed for the isolation of myxobacteria. Due to the low efficiency of the majority of conventional DNA extraction techniques, for molecular identification of the strain Streptomyces sp. Act4Zk, a new technique for DNA extraction of Actinobacteria was developed. In order to explore potential bioactivities of the strain, extracts of the fermented broth culture were prepared by an organic solvent (i.e., ethyl acetate) extraction method using. These ethyl acetate extracts were subjected to HPLC fractionation against standard microorganisms, followed by LC/MS analysis. Based on morphological, physiological, biochemical, and 16S rRNA gene sequence data, strain Streptomyces sp. Act4Zk is likely to be a new species of Streptomyces, close to Streptomyces genecies and Streptomyces roseolilacinus. Antimicrobial assay indicated high antifungal activity as well as antibacterial activity against Mycobacterium smegmatis and Gram-positive bacteria for the new strain. HPLC and LC/MS analyses of the extracts led to the identification of three different compounds and confirmed our hypothesis that the interesting species of the genus Streptomyces being a good producer of staurosporine and some derivatives.

中文翻译：

链霉菌属的分离和鉴定。Act4Zk，星形孢菌素和一些衍生物的良好生产商

在本研究中，菌株 Streptomyces sp。Act4Zk 是基于为分离粘细菌而开发的方法分离的。由于大多数常规 DNA 提取技术的低效率，用于菌株 Streptomyces sp. 的分子鉴定。Act4Zk 是一种提取放线菌 DNA 的新技术。为了探索该菌株的潜在生物活性，使用有机溶剂（即乙酸乙酯）提取方法制备发酵肉汤培养物的提取物。这些乙酸乙酯提取物针对标准微生物进行 HPLC 分级，然后进行 LC/MS 分析。基于形态学、生理学、生化和 16S rRNA 基因序列数据，菌株 Streptomyces sp. Act4Zk 很可能是链霉菌属的新种，接近 Streptomyces 基因和 Streptomyces roseolilacinus。抗菌测定表明新菌株具有高抗真菌活性以及对耻垢分枝杆菌和革兰氏阳性菌的抗菌活性。提取物的 HPLC 和 LC/MS 分析导致鉴定了三种不同的化合物，并证实了我们的假设，即链霉菌属的有趣物种是星形孢菌素和一些衍生物的良好生产者。

更新日期：2020-11-10

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