In the present study, the effect of nerve stimulation on the secretory activity of the ovary of adult females was analyzed for the first time in amphibians. Results revealed that in Rhinella arenarum the stimulation of nerves that supply the gonad induced an increase in estradiol and progesterone secretion, this response showing differences during the reproductive cycle of the species. During the postreproductive period, an increase in estradiol secretion was observed while, in the reproductive period, progesterone secretion increased. Our results suggest that the sympathetic division of the autonomic nervous system would be responsible for this increase, taking into account that, under our experimental conditions, acetylcholine did not affect the endocrine activity of the gonad, while adrenaline (epinephrine) was effective in inducing steroid secretion an effect that could be due to interaction with β receptors. On the other hand, our data show that the association of adrenaline with follicle‐stimulating hormone increased estradiol secretion during the postreproductive period, while the association of catecholamine with LH or hCG increased progesterone secretion during the reproductive period. Our results would suggest that nerve stimulation, mediated by the release of adrenaline, would act synergistically with gonadotrophins to stimulate steroid secretion.

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神经刺激对两栖动物卵巢类固醇分泌的影响

在本研究中，首次在两栖动物中分析了神经刺激对成年女性卵巢分泌活性的影响。结果显示，在莱茵兰竞技场提供性腺的神经刺激导致雌二醇和孕酮分泌增加，这种反应显示出该物种生殖周期的差异。在生殖后期间，观察到雌二醇分泌增加，而在生殖期间，孕酮分泌增加。我们的结果表明，自主神经系统的交感神经分裂是造成这种增加的原因，考虑到在我们的实验条件下，乙酰胆碱不会影响性腺的内分泌活性，而肾上腺素（肾上腺素）可以有效诱导类固醇分泌可能是由于与β受体相互作用引起的。另一方面，我们的数据显示，肾上腺素与促卵泡激素的结合在生殖后期增加了雌二醇的分泌，而儿茶酚胺与LH或hCG的结合在生殖期增加了黄体酮的分泌。我们的结果表明，由肾上腺素释放介导的神经刺激将与促性腺激素协同作用以刺激类固醇分泌。