The influence of toluene alone and in combination with plant polyphenols apigenin, daidzein or rutin on viability, proliferation (proliferating cell nuclear antigen accumulation), apoptosis (Bax accumulation) and release of progesterone (P), testosterone (T) and estradiol (E) in cultured porcine ovarian granulosa cells was evaluated. Toluene reduced ovarian cell viability, proliferation and E release; it promoted P release, demonstrating no effect on apoptosis or T output. Apigenin alone failed to affect cell viability, proliferation, apoptosis and P and T release, but stimulated E release, promoting the inhibitory action of toluene on proliferation, preventing and even reversing the stimulatory effect of toluene on apoptosis and P. Daidzein alone reduced cell viability and promoted T release, preventing and reversing the stimulatory effect of toluene on cell proliferation. Rutin administration reduced cell viability and E output, promoting the inhibitory action of toluene on cell viability and stimulatory effect on P release, and preventing the inhibitory action of toluene on E release. Toluene reduced apigenin‐ and rutin‐induced E release, promoting action of daidzein on cell viability. These observations suggest the action of toluene and plant polyphenols on ovarian cell functions and the functional interrelationships between these molecules in the ovary.

中文翻译：

---

植物多酚可直接影响卵巢细胞功能并改变甲苯作用

甲苯单独和与植物多酚芹菜素、黄豆苷元或芦丁组合对活力、增殖（增殖细胞核抗原积累）、细胞凋亡（Bax 积累）和孕酮 (P)、睾酮 (T) 和雌二醇 (E) 释放的影响在培养的猪卵巢颗粒细胞中进行了评价。甲苯降低卵巢细胞活力、增殖和 E 释放；它促进 P 释放，表明对细胞凋亡或 T 输出没有影响。单独使用芹菜素不影响细胞活力、增殖、凋亡和P、T释放，但刺激E释放，促进甲苯对增殖的抑制作用，阻止甚至逆转甲苯对细胞凋亡和P的刺激作用。单独使用大豆苷元降低细胞活力并推广T发布，防止和逆转甲苯对细胞增殖的刺激作用。芦丁给药降低细胞活力和E释放，促进甲苯对细胞活力的抑制作用和对P释放的刺激作用，阻止甲苯对E释放的抑制作用。甲苯减少芹菜素和芦丁诱导的 E 释放，促进黄豆苷元对细胞活力的作用。这些观察结果表明甲苯和植物多酚对卵巢细胞功能的作用以及这些分子在卵巢中的功能相互关系。甲苯减少芹菜素和芦丁诱导的 E 释放，促进黄豆苷元对细胞活力的作用。这些观察结果表明甲苯和植物多酚对卵巢细胞功能的作用以及这些分子在卵巢中的功能相互关系。甲苯减少芹菜素和芦丁诱导的 E 释放，促进黄豆苷元对细胞活力的作用。这些观察结果表明甲苯和植物多酚对卵巢细胞功能的作用以及这些分子在卵巢中的功能相互关系。