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The ESCRT‐I components VPS28A and VPS28B are essential for auxin‐mediated plant development
The Plant Journal ( IF 6.2 ) Pub Date : 2020-10-15 , DOI: 10.1111/tpj.15024 Jianyang Liu 1, 2 , Yanning Wang 1, 2 , Youfa Cheng 1, 2, 3
The Plant Journal ( IF 6.2 ) Pub Date : 2020-10-15 , DOI: 10.1111/tpj.15024 Jianyang Liu 1, 2 , Yanning Wang 1, 2 , Youfa Cheng 1, 2, 3
Affiliation
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The highly conserved endosomal sorting complex required for transport (ESCRT) pathway plays critical roles in endosomal sorting of ubiquitinated plasma membrane proteins for degradation. However, the functions of many components of the ESCRT machinery in plants remain unsolved. Here we show that the ESCRT‐I subunits VPS28A and VPS28B are functionally redundant and required for embryonic development in Arabidopsis. We conducted a screen for genetic enhancers of pid, which is defective in auxin signaling and transport. We isolated a no‐‐cotyledon in pid 104 (ncp104) mutant, which failed to develop cotyledons in a pid background. We discovered that ncp104 was a unique recessive gain‐of‐function allele of vps28a. VPS28A and VPS28B were expressed during embryogenesis and the proteins were localized to the trans‐Golgi network/early endosome and post‐Golgi/endosomal compartments, consistent with their functions in endosomal sorting and embryogenesis. The single vps28a and vps28b loss‐of‐function mutants did not display obvious developmental defects, but their double mutants showed abnormal cell division patterns and were arrested at the globular embryo stage. The vps28a vps28b double mutants showed altered auxin responses, disrupted PIN1‐GFP expression patterns, and abnormal PIN1‐GFP accumulation in small aberrant vacuoles. The ncp104 mutation may cause the VPS28A protein to become unstable and/or toxic. Taken together, our findings demonstrate that the ESCRT‐I components VPS28A and VPS28B redundantly play essential roles in vacuole formation, endosomal sorting of plasma membrane proteins, and auxin‐mediated plant development.
中文翻译：
ESCRT-I组件VPS28A和VPS28B对于生长素介导的植物发育至关重要
运输（ESCRT）途径所需的高度保守的内体分选复合物在泛素化质膜蛋白降解的内体分选中起关键作用。但是，工厂中ESCRT机械的许多组件的功能仍未解决。在这里我们显示ESCRT-1亚基VPS28A和VPS28B在功能上是多余的，是拟南芥胚胎发育所必需的。我们对pid的基因增强子进行了筛选，该基因在生长素的信号传导和运输中存在缺陷。我们分离出一种Ñ O-- Ç在otyledon p ID 104（ncp104）突变体，其未能发展在子叶PID背景。我们发现ncp104是vps28a的独特隐性功能获得性等位基因。VPS28A和VPS28B在胚胎发生过程中表达，并且蛋白质定位于反高尔基体网络/早期内体和高尔基体后/内体区室，与其在内体分选和胚胎发生中的功能一致。vps28a和vps28b功能丧失的单个突变体没有显示出明显的发育缺陷，但它们的双重突变体却显示出异常的细胞分裂模式，并被停滞在球形胚阶段。该vps28b vps28a双突变体显示改变生长素反应，破坏PIN1-GFP表达模式，并在小液泡异常异常PIN1-GFP积累。的ncp104突变可能导致VPS28A蛋白变得不稳定和/或有毒。综上所述，我们的研究结果表明，ESCRT-I成分VPS28A和VPS28B在液泡形成，质膜蛋白的内体分选以及生长素介导的植物发育中起着多余的作用。
更新日期：2020-12-22
中文翻译：
ESCRT-I组件VPS28A和VPS28B对于生长素介导的植物发育至关重要
运输（ESCRT）途径所需的高度保守的内体分选复合物在泛素化质膜蛋白降解的内体分选中起关键作用。但是，工厂中ESCRT机械的许多组件的功能仍未解决。在这里我们显示ESCRT-1亚基VPS28A和VPS28B在功能上是多余的，是拟南芥胚胎发育所必需的。我们对pid的基因增强子进行了筛选，该基因在生长素的信号传导和运输中存在缺陷。我们分离出一种Ñ O-- Ç在otyledon p ID 104（ncp104）突变体，其未能发展在子叶PID背景。我们发现ncp104是vps28a的独特隐性功能获得性等位基因。VPS28A和VPS28B在胚胎发生过程中表达，并且蛋白质定位于反高尔基体网络/早期内体和高尔基体后/内体区室，与其在内体分选和胚胎发生中的功能一致。vps28a和vps28b功能丧失的单个突变体没有显示出明显的发育缺陷，但它们的双重突变体却显示出异常的细胞分裂模式，并被停滞在球形胚阶段。该vps28b vps28a双突变体显示改变生长素反应，破坏PIN1-GFP表达模式，并在小液泡异常异常PIN1-GFP积累。的ncp104突变可能导致VPS28A蛋白变得不稳定和/或有毒。综上所述，我们的研究结果表明，ESCRT-I成分VPS28A和VPS28B在液泡形成，质膜蛋白的内体分选以及生长素介导的植物发育中起着多余的作用。
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