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A Novel Amperometric Glucose Biosensor Based on Fe3O4‐Chitosan‐β‐Cyclodextrin/MWCNTs Nanobiocomposite
Electroanalysis ( IF 2.7 ) Pub Date : 2020-10-14 , DOI: 10.1002/elan.202060399
Lincai Peng 1 , Yingjie Luo 1 , Hongping Xiong 2 , Shun Yao 1 , Minghui Zhu 2 , Hang Song 1
Affiliation  

A novel enzymatic biosensing platform toward glucose is achieved with nanocomposite of magnetic nanoparticles (Fe3O4−CS−CD) and multi‐walled carbon nanotubes (MWCNTs). The synergistic effect of chitosan, β‐cyclodextrin and MWCNTs can facilitate electron transfer between enzyme and electrode based on the promoting results of the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The new biosensors exhibited direct electron transfer (DET) from enzyme to electrode after glucose oxidase (GOx) was immobilized on the modified electrode with the nanocomposite. Consequently, the enzymatic glucose biosensor displayed a considerably wide linear range (40 μM to 1.04 mM) with a high sensitivity of 23.59 μA mM−1cm−2, low detection limit of 19.30 μM, good selectivity, reproducibility and repeatability for detecting glucose. In addition, the current response still retained at 93.4 % after 25 days. Furthermore, the practical application of glucose biosensor was test in human serum samples with satisfactory accuracy, demonstrating promising and practical potential in biomedical diagnostics.

中文翻译:

基于Fe3O4-壳聚糖-β-环糊精/ MWCNTs纳米生物复合物的新型安培葡萄糖生物传感器

利用磁性纳米颗粒(Fe 3 O 4 -CS-CD)和多壁碳纳米管(MWCNT)的纳米复合材料,实现了一种针对葡萄糖的新型酶生物传感平台。基于循环伏安法(CV)和电化学阻抗谱(EIS)的促进结果,壳聚糖,β-环糊精和MWCNT的协同作用可促进酶与电极之间的电子转移。在将葡萄糖氧化酶(GOx)固定在具有纳米复合材料的修饰电极上之后,新的生物传感器显示出从酶到电极的直接电子转移(DET)。因此,酶促葡萄糖生物传感器显示出相当宽的线性范围(40μM至1.04 mM),具有23.59μAmM -1 cm -2的高灵敏度。,检测限低至19.30μM,具有良好的选择性,重现性和可重复性,可检测葡萄糖。此外,目前的反应在25天后仍保持在93.4%。此外,葡萄糖生物传感器的实际应用以令人满意的准确性在人血清样品中进行了测试,证明了在生物医学诊断中有希望和实用的潜力。
更新日期:2020-10-14
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