Objectives

The toxicity of cadmium in various animal models, in particular the liver, has been studied. The objective of this research was to determine the antioxidant and the hepatoprotective role of Pleurotus ostreatus (PE) extract in Cadmium-intoxicated rats.

Methods

Rats of the first group were used as control animals while rats of the second group were administered PE for 5 days. Animals of the third group were served as Cadmium intoxicated group and finally, rats of the fourth-group were treated orally with PE afterwards; cadmium was administered over 5 consecutive days. By using atomic absorption, the Cadmium concentration was measured in the liver. Hepatic histological changes were estimated through examination of stained tissue sections. The oxidative status in the liver was investigated through determining the concentration of glutathione (GSH), catalase (CAT), malondialdehyde (MDA) and the reactive oxygen species (ROS) in the liver homogenate. Finally the expression of Nrf2-mRNA was determined using real time PCR.

Results

Cadmium induced major hepatic histopathological damage, elevated levels of cadmium, and hepatic oxidative stress where the concentration GSH, CAT, MDA and ROS were affected in the liver. Additionally, cadmium administration resulted in substantial up-regulation of Nrf2-mRNA expression. Moreover, PE attenuated the hepatic cadmium intoxication where it improved the histological impairments and the level of accumulated cadmium in liver tissue was decreased. Likewise, the treatment attenuated the oxidative stress and down-regulated the expression of Nrf2.

Conclusions

Our results indicated that PE is associated with significant antioxidant and anti-inflammatory activity and alleviated the hepatotoxicity induced by cadmium chloride.

中文翻译：

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平菇提取物对镉中毒大鼠的肝保护作用

目标

已经研究了镉在各种动物模型，特别是肝脏中的毒性。这项研究的目的是确定平菇平菇提取物在镉中毒大鼠中的抗氧化剂和对肝脏的保护作用。

方法

将第一组的大鼠用作对照动物，而第二组的大鼠给予PE 5天。将第三组动物作为镉中毒组，最后将第四组的大鼠口服PE。连续5天施用镉。通过原子吸收，测量肝脏中的镉浓度。通过检查染色的组织切片估计肝组织学变化。通过确定肝脏匀浆中谷胱甘肽（GSH），过氧化氢酶（CAT），丙二醛（MDA）和活性氧（ROS）的浓度来研究肝脏中的氧化状态。最后，使用实时PCR确定Nrf2-mRNA的表达。

结果

镉可引起主要的肝脏组织病理学损害，镉水平升高和肝脏氧化应激，其中肝脏中GSH，CAT，MDA和ROS的浓度受到影响。另外，镉的施用导致Nrf2-mRNA表达的显着上调。此外，PE减轻了肝镉中毒，改善了组织学损伤，并降低了肝组织中积累的镉水平。同样，该处理减弱了氧化应激并下调了Nrf2的表达。

结论

我们的结果表明，PE与显着的抗氧化剂和抗炎活性有关，并减轻了氯化镉引起的肝毒性。