According to the mechanism of hydrazine to ester bond elimination, a novel ratiometric fluorescent probe (FCP) based on the fluorescein-coumarin structure is designed and synthesized for detecting hydrazine. The obvious red shift in the absorption and fluorescence spectrum is caused by the hydrolysis of the ester bond of FCP by upon mixing with hydrazine. The proposed FCP probe is shown to have linear detection ranges from 0 to 250 nM for hydrazine, and LOD is 0.364 nM. In addition, this promising probe possesses high sensitivity and selectivity for monitoring the intracellular hydrazine, thus, has great potential to be applied in early diagnosis of disease. Meanwhile, it has been successfully applied to the detection and cell imaging of hydrazine in actual water samples.

根据肼消除酯键的机理，设计合成了一种基于荧光素-香豆素结构的新型比例荧光探针（FCP），用于检测肼。吸收和荧光光谱中明显的红移是由FCP与肼混合水解后的酯键引起的。所提出的FCP探针对肼的线性检测范围为0至250 nM，LOD为0.364 nM。另外，该有前途的探针具有用于监测细胞内肼的高灵敏度和选择性，因此，在疾病的早期诊断中具有很大的潜力。同时，已成功应用于实际水样中肼的检测和细胞成像。