Little is known about the intrinsic specification of postnatal cerebellar neural stem cells (NSCs) and to what extent they depend on information from their local niche. Here, we have used an adapted cell preparation of isolated postnatal NSCs and live imaging to demonstrate that cerebellar progenitors maintain their neurogenic nature by displaying hallmarks of NSCs. Furthermore, by using this preparation, all the cell types produced postnatally in the cerebellum, in similar relative proportions to those observed in vivo, can be monitored. The fact that neurogenesis occurs in such organized manner in the absence of signals from the local environment, suggests that cerebellar lineage progression is to an important extent governed by cell-intrinsic or pre-programmed events. Finally, we took advantage of the absence of the niche to assay the influence of the vesicular nucleotide transporter inhibition, which dramatically reduced the number of NSCs in vitro by promoting their progression toward neurogenesis.

对出生后小脑神经干细胞 (NSC) 的内在特性以及它们在多大程度上依赖于来自其本地生态位的信息知之甚少。在这里，我们使用了分离的出生后 NSC 和实时成像的适应性细胞制备，以证明小脑祖细胞通过显示 NSC 的标志来保持其神经源性。此外，通过使用这种制剂，出生后在小脑中产生的所有细胞类型与体内观察到的细胞类型的相对比例相似，可以监控。在没有来自局部环境的信号的情况下，神经发生以这种有组织的方式发生的事实表明，小脑谱系进展在很大程度上受细胞内在或预编程事件的控制。最后，我们利用生态位的缺失来测定囊泡核苷酸转运蛋白抑制的影响，这通过促进它们向神经发生的进展显着减少了体外NSC 的数量。