Bacopa monnieri (Linn.) Wettst. commonly known as waterhyssop, Brahmi plant, traditionally used for memory enhancement, nerve tonic, epilepsy, central nervous system (CNS), antidepressant, anxiety, blood pressure and antioxidant activities. Due to pharmaceutical demands its lost natural habitat. At this juncture we describe a resourceful protocol for micropropagation of water hyssop plant. Surface sterilized leaf and nodal explants were inoculated on basal MS semi-solid medium added with PGRs; auxins, cytokinins. Highest calli formation from leaf explants was obtained on NAA (2.5 mg⁻¹) and showed (94.22%) accompanied via 2,4-D showed (2.5 mg⁻¹; 82.43%), maximum calli formation in nodal explants was obtained on 2,4-D showed (2.5 mg⁻¹; 71.14%) followed by NAA (2.5 mg⁻¹) showed (62.15%), in internodes explants uppermost calli formation was obtained from 2,4-D showed (2.5 mg⁻¹; 65.21%) followed by NAA (2.5 mg⁻¹) showed (52.14%). The maximum somatic embryogenic callus, calli induction and formation (84%) was observed on 2,4-D + KIN (2.0 + 1.5 mg⁻¹) amended solid medium. Uppermost shoot formation was observed in combination of IAA + BAP (1.0 + 1.0 mg⁻¹) showed (78.54%) shoot formation followed by IBA (2.0 mg⁻¹) alone showed (75.37%). The maximum shoot elongation was noticed from NAA + BAP (3.0 + 3.0 mg⁻¹) with 21.21 cm followed by NAA (2.0 mg⁻¹) showed (15.22 cm) although, chief root formation was obtained from IBA (2.0 mg⁻¹) with 83.75% root formation along higher number of roots (47.43%) per shoot. Followed by IAA (2.0 mg⁻¹) showed root induction (73.43%) and no of roots (38.54%) per shoot. In hardening under pot condition plants survivability (100%) was observed under glass house conditions, the present in vitro PTC techniques is extremely significant to gratifying its natural conservation.

Bacopa monnieri（林恩）。通常被称为waterhyssop，婆罗米植物，传统上用于增强记忆力，神经补品，癫痫病，中枢神经系统（CNS），抗抑郁剂，焦虑症，血压和抗氧化活性。由于药物需求，其失去了自然栖息地。在此关头，我们描述了一种用于水牛膝草微繁殖的资源丰富的协议。在添加了PGR的基础MS半固体培养基上接种表面灭菌的叶片和节点外植体。生长素，细胞分裂素。在NAA上，叶外植体的愈伤组织形成最高（2.5 mg ^-1），并显示（94.22％），通过2,4-D示（2.5 mg ^-1； 82.43％），在节点上的外植体中愈伤组织的形成最大。 ，4-D示（2.5 mg ^-1; 71.14％），随后显示出NAA（2.5 mg ^-1）（62.15％），在节点间外植体中，从2,4-D显示出最高的愈伤组织形成（2.5 mg ^-1 ; 65.21％），其次是NAA（2.5 mg ^-1））（52.14％）。在2,4-D + KIN（2.0 + 1.5 mg ^-1）改良固体培养基上观察到最大的体细胞胚发生愈伤组织，愈伤组织诱导和形成（84％）。在IAA + BAP（1.0 + 1.0 mg ^-1）的组合中观察到最高的枝条形成（78.54％），随后是仅IBA（2.0 mg ^-1）的枝条形成（75.37％）。从NAA + BAP（3.0 + 3.0 mg ^-1）到21.21 cm，然后是NAA（2.0 mg ^-1）注意到最大的芽伸长。）显示（15.22 cm），但主根形成是从IBA（2.0 mg ^-1）获得的，其中根形成率为83.75％，且每枝的根数更高（47.43％）。其次是IAA（2.0 mg ^-1），每芽显示有根诱导（73.43％），无根（38.54％）。在盆栽条件下的植物硬化中，在温室条件下可观察到植物的存活率（100％），目前的体外PTC技术对于满足其自然保存极为重要。