The isolation and identification of beneficial bacteria from the active phase of composting is considered to be a key bio-quality parameter for the assessment of the process. The aim of this work was the selection and identification of beneficial bacteria from a co-composting experiment of vegetable waste (VW), olive oil mill waste (O₂MW), and phosphate sludge (PS). Phosphate-solubilizing strains were isolated from the thermophilic phase using Pikovskaya (PVK) solid medium supplemented with tricalcium phosphate Ca3(PO4) (TCP) as the sole source of phosphorus (P). Therefore, the selected isolate Alcaligenes aquatilis GTE53 was tested to tolerate abiotic stresses (different levels of temperature, variable pH, high salinity and water stress). The isolate was also assessed for indole acetic acid (IAA) and siderophores synthesis, nitrogen fixation, phenol degradation and pathogens inactivation. The quality of the co-composting process was also investigated by monitoring the physico-chemical parameters. The obtained results showed that A. aquatilis GTE53 displayed a higher solubilization index of 2.4 and was efficiently dissolved, up to 162.8 and 247.4 mg·mL⁻¹ of inorganic phosphate from PS and phosphate rock (PR), respectively. A. aquatilis GTE53 exhibited siderophores and IAA release, along with atmospheric nitrogen fixation. In addition to that, A. aquatilis GTE53 showed a high resistance to heat and tolerance to acidic and alkaline pH, high salinity and water stress. Moreover, A. aquatilis GTE53 could degrade 99.2% of phenol from a high-concentrated medium (1100 mg·L⁻¹ of phenol) and can inactivate the most abundant pathogens in industrial wastes: Escherichia coli, Streptococcus sp., Salmonella sp., and Fusarium oxysporum albedinis. Analysis of temperature, pH, electrical conductivity, carbon/nitrogen (C/N) ratio, indicated successful co-composting. An efficient transformation of P to the available form and a great abatement of polyphenols, were also recorded during the process. The findings of this study will help to advance the understanding of A. aquatilis GTE53 functions and will facilitate its application to promote beneficial microbial organisms during composting, thus obtaining a high-quality product.

从堆肥活性阶段分离和鉴定有益细菌被认为是评估该过程的关键生物质量参数。这项工作的目的是从蔬菜废物（VW），橄榄油厂废物（O ₂ MW）和磷酸盐污泥（PS）的共同堆肥实验中选择和鉴定有益细菌。使用补充磷酸三钙Ca3（PO4）（TCP）作为唯一磷（P）的Pikovskaya（PVK）固体培养基从嗜热相中分离出可溶解磷酸盐的菌株。因此，选择的分离水生产碱菌测试GTE53可以耐受非生物胁迫（不同水平的温度，可变的pH，高盐度和水分胁迫）。还评估了分离物的吲哚乙酸（IAA）和铁载体合成，固氮，苯酚降解和病原体灭活的能力。还通过监测理化参数研究了堆肥过程的质量。将所得到的结果表明，A. aquatilis GTE53显示2.4的更高溶解指数和被有效溶解，高达162.8 247.4和毫克·ML ^-1从PS分别无机磷酸盐和磷酸盐岩（PR），的。A. aquatilis GTE53表现出铁载体和IAA释放，以及大气固氮作用。除此之外，A。aquatilisGTE53表现出很高的耐热性和对酸性和碱性pH的耐受性，高盐度和水分胁迫。此外，A. aquatilis GTE53可以从高浓介质降解苯酚99.2％（1100毫克·L ^-1苯酚的），并且可以在灭活工业废料中最丰富的病原体：大肠杆菌，链球菌。菌属，沙门氏菌。SP，和尖孢镰刀白化病。对温度，pH，电导率，碳/氮（C / N）比的分析表明成功了堆肥。在此过程中，还记录了P高效转化为可用形式和大量减少的多酚。这项研究的结果将有助于推进的理解A. aquatilis GTE53功能，将促进其应用堆肥，从而获得高品质的产品过程中促进有益微生物。