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Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice
Reproductive Toxicology ( IF 3.3 ) Pub Date : 2020-10-15 , DOI: 10.1016/j.reprotox.2020.10.007
Shuxian Wang 1 , Qiwei Chen 2 , Yu Zhang 3 , Feng Zheng 4 , Tongmin Xue 5 , Xie Ge 1 , Rujun Ma 1 , Xiaoyan Li 1 , Ronghua Wu 1 , Kuan Liang 2 , Zhang Qian 1 , Yifeng Ge 1 , Jinzhao Ma 1 , Bing Yao 6
Affiliation  

Hydrogen sulfide (H2S), a gaseous intracellular signal transducer, participates in multiple physiological and pathological conditions, including reproductive conditions, and disrupts spermatogenesis. The blood-testis barrier (BTB) plays a vital role in spermatogenesis. However, the effect of H2S on the BTB and the underlying mechanism remain unclear. Herein, we examined the effect of H2S and omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on the BTB and testicular functions. ICR male mice were randomly divided into the following groups: control, H2S exposure, and H2S exposure with ω-3 PUFAs intervention. The sperm parameters (sperm concentration and sperm motility) declined in the H2S group and improved in the ω-3 intervention group. BTB integrity was severely disrupted by H2S, and the BTB-related gene levels (ZO-1, Occludin, Claudin 11) decreased; ω-3 supplementation could alleviate BTB disruption by upregulating BTB-related genes, and TM4 Sertoli cells had a similar trend in vitro. p38 MAPK phosphorylation was upregulated in the Na2S treatment group and downregulated after ω-3 cotreatment. These findings suggest that H2S can impair the BTB and that ω-3 PUFAs supplementation can attenuate H2S toxicity in the male reproductive system. Our study elucidated the relationship between a gasotransmitter (H2S) and the BTB and identified the potential therapeutic effect of ω-3 PUFAs.



中文翻译:

Omega-3 多不饱和脂肪酸减轻硫化氢引起的成年小鼠睾丸血睾屏障破坏

硫化氢 (H2S) 是一种气态细胞内信号转换器,参与多种生理和病理条件,包括生殖条件,并破坏精子发生。血睾屏障 (BTB) 在精子发生中起着至关重要的作用。然而,H 2 S 对BTB 的影响及其潜在机制仍不清楚。在此,我们研究了 H 2 S 和 omega-3 多不饱和脂肪酸 (ω-3 PUFA) 对 BTB 和睾丸功能的影响。ICR 雄性小鼠随机分为以下组:对照组、H 2 S 暴露组和具有ω-3 PUFAs 干预的H 2 S 暴露组。精子参数(精子浓度和精子活力)在 H 2S组并在ω-3干预组中有所改善。BTB完整性被H 2 S严重破坏,BTB相关基因水平(ZO-1、Occludin、Claudin 11)降低;补充 ω-3 可以通过上调 BTB 相关基因来减轻 BTB 破坏,TM4 Sertoli 细胞在体外也有类似的趋势。p38 MAPK 磷酸化在 Na 2 S 处理组中上调,在 ω-3 共处理后下调。这些发现表明 H 2 S 可以损害 BTB,补充 ω-3 PUFA 可以减弱男性生殖系统中的H 2 S 毒性。我们的研究阐明了气体发射器 (H 2S) 和 BTB,并确定了 ω-3 PUFA 的潜在治疗效果。

更新日期:2020-10-15
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