Abstract F-box proteins constitute one of the largest protein families and play many important roles in plant. To date, less is known about functions of wheat F-box protein. A Kelch-type F-box gene TaFBK was isolated from wheat TcLr15. TaFBK transcripts were more abundant in pistil and leaf tissues, and accumulated more in the susceptible combination than that in the resistant one. The treatment of SA, MeJA, NaCl and PEG caused a slight up-regulation of TaFBK. GFP localization studies showed that TaFBK accumulates in the nucleus and cytoplasm. Moreover, TaFBK bound TaSkp1 by the F-box domain and recognized TaSLY1 via the Kelch domain.

中文翻译：

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一种新型小麦 F-box/Kelch 基因 TaFBK 相互作用伙伴的分子鉴定和获得

摘要 F-box蛋白是最大的蛋白质家族之一，在植物中发挥着重要作用。迄今为止，人们对小麦 F-box 蛋白的功能知之甚少。从小麦 TcLr15 中分离出 Kelch 型 F-box 基因 TaFBK。TaFBK 转录物在雌蕊和叶组织中更丰富，并且在易感组合中比在抗性组合中积累更多。SA、MeJA、NaCl 和 PEG 的处理导致 TaFBK 的轻微上调。GFP 定位研究表明 TaFBK 在细胞核和细胞质中积累。此外，TaFBK 通过 F-box 域结合 TaSkp1，并通过 Kelch 域识别 TaSLY1。