To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

中文翻译：

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NMDA 型 GluRs 与 GluN1 和 GluN2B 抗体的内化特征

为了表征 NMDA 型 GluRs 抗体对 NMDA 型谷氨酸受体 (GluRs) 的内化，我们制备了针对人 GluN1 和 GluN2B 的 N 端的兔抗体，并使用共聚焦显微镜检查了 HEK293T 细胞中 NMDA 型 GluRs 的内化。NMDA 型 GluR 的内化发生在与 GluN1 和/或 GluN2B 抗体孵育后至少 10 分钟，并且与温度有关。这些发现证实，存在于 NMDAR 脑炎患者脑脊液中的针对 GluN1 和 GluN2B N 末端的抗体可介导 NMDA 型 GluR 复合物的迅速内化。