The fermentative production of biobased chemicals and polymers using crude lignocellulose hydrolysates is challenging due to the presence of various inhibitory compounds and multiple sugars. This study evaluates the metabolic response of Actinobacillus succinogenes for the production of succinic acid using spent sulphite liquor (SSL) as feedstock derived from industrial acidic sulphite pulping of Eucalyptus globulus hardwood. A transcriptomic approach led to significant insights on gene regulation of the major metabolic pathways (glycolysis, pentose phosphate pathway, TCA cycle, pyruvate metabolism and oxidative phosphorylation) in batch cultures carried out on SSL and compared with glucose and xylose. Significantly overexpressed genes in SSL compared to glucose and xylose were fructose biphosphate aldolase (> 1.18-fold change) in the catabolism, phosphoenolpyruvate carboxykinase (> 1.59-fold change) and malate dehydrogenase (> 1.49-fold change) in the TCA cycle, citrate lyase (> 1.7-fold change), dihydrolipoamide dehydrogenase (> 0.88-fold change), pyruvate dehydrogenase E2 (> 1.63-fold change) and pyruvate formate lyase (> 0.61-fold change), involved in acetyl-CoA pathways. Finally, C4 tricarboxylic transporters were overexpressed (DCU (> 1.61-fold change) and 0079 (> 4.19-fold change). SSL was responsible for the upregulation of genes involved in the TCA cycle and oxidative phosphorylation, while xylose showed similar results with SSL in the oxidative phosphorylation.

由于存在各种抑制性化合物和多种糖，使用粗木质纤维素水解物发酵生产生物基化学品和聚合物具有挑战性。本研究评估了产琥珀酸放线杆菌的代谢反应，该反应使用亚硫酸盐废液 (SSL) 作为来自蓝桉工业酸性亚硫酸盐制浆的原料硬木。转录组学方法对在 SSL 上进行的分批培养中主要代谢途径（糖酵解、戊糖磷酸途径、TCA 循环、丙酮酸代谢和氧化磷酸化）的基因调控产生了重要见解，并与葡萄糖和木糖进行了比较。与葡萄糖和木糖相比，SSL 中显着过表达的基因是分解代谢中的果糖二磷酸醛缩酶（> 1.18 倍变化）、TCA 循环中的磷酸烯醇丙酮酸羧激酶（> 1.59 倍变化）和苹果酸脱氢酶（> 1.49 倍变化），柠檬酸裂解酶（> 1.7 倍变化）、二氢硫辛酰胺脱氢酶（> 0.88 倍变化）、丙酮酸脱氢酶 E2（> 1.63 倍变化）和丙酮酸甲酸裂解酶（> 0.61 倍变化），参与乙酰辅酶 A 途径。最后，