To reduce the largemouth bass ulcer syndrome (LBUSV) aquatic economic losses, it must take effective preventive measures and coping strategies should be urgently investigated. In this research, the effects of a functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for DNA vaccine administration in largemouth bass (Micropterus Salmoides) against LBUSV were studied. Our results showed that SWCNTs loaded with DNA vaccine induced a better protection to largemouth bass against LBUSV. We found more than 10 times increase in serum antibody levels, enzyme activities and immune-related genes (IL-6, IL-8, IFN-γ, IgM and TNF-α) expression, in the SWCNTs-pcDNA-MCP immunized groups compared with PBS group and the pure SWCNTs group. The survival rates for control group (PBS), pure SWCNTs groups (40 mg L⁻¹), four pcDNA-MCP groups (5 mg L⁻¹, 10 mg L⁻¹, 20 mg L⁻¹ and 40 mg L⁻¹) and four SWCNTs-pcDNA-MCP groups (5 mg L⁻¹, 10 mg L⁻¹, 20 mg L⁻¹ and 40 mg L⁻¹) were 0%, 0%, 0%, 2.77%, 11.11%, 19.44%, 27.78%, 38.89%, 52.78% and 61.11%, respectively. Our results demonstrate that the SWCNTs-DNA vaccine can be used as a new method against LBUSV showing protection following challenge with LBUSV.

为减少大嘴鲈鱼溃疡综合症（LBUSV）的水生经济损失，必须采取有效的预防措施，应紧急研究应对策略。在这项研究中，研究了功能化的单壁碳纳米管（SWCNT）用作大口黑鲈（Micropterus Salmoides）对抗LBUSV的DNA疫苗给药的载体。我们的结果表明，装有DNA疫苗的SWCNT可以更好地保护大嘴鲈免受LBUSV侵害。我们发现在SWCNTs-pcDNA- MCP中，血清抗体水平，酶活性和免疫相关基因（IL-6，IL-8，IFN-γ，IgM和TNF-α）的表达增加了10倍以上免疫组与PBS组和纯SWCNTs组相比。存活率为对照组（PBS），纯的SWCNT组（40毫克的L ^-1），四个pcDNA- MCP组（5毫克的L ^-1，10毫克的L ^-1，20毫克的L ^-1和40毫克的L ^-1）和四个的SWCNT-pcDNA- MCP组（5毫克的L ^-1，10毫克的L ^-1，20毫克的L ^-1和40毫克的L ^-1）分别为0％，0％，0％，2.77％，11.11％，分别为19.44％，27.78％，38.89％，52.78％和61.11％。我们的结果表明，SWCNTs-DNA疫苗可作为抗LBUSV的新方法，显示出在LBUSV攻击后具有保护作用。