Abstract A degradable and water-soluble polyphosphoester (PPE), namely poly(methyl ethylene phosphate)-dimethacrylate (PMEP-DMA), was synthesized and crosslinked by UV irradiation to prepare PPE-hydrogels. Hydrogels with 10 and 15 wt% of PMEP were prepared after UV-irradiation with an additional 0.2 wt% of photoinitiator. The colorless and transparent PPE hydrogels were studied for their swelling and water uptake. The rheological analysis demonstrated their viscoelastic behavior. The PPE hydrogels were compared to poly(ethylene glycol) (PEG) hydrogels prepared from PEG-macromonomers of similar degrees of polymerization. Hydrolysis experiments proved a successful disintegration of the PPE hydrogels compared to PEG analogs; a faster weight loss for the hydrogels with 10 wt% of PMEP compared to the 15 wt% hydrogels was detected. NMR spectroscopy further proved the release of soluble PPEs from the network and the formation of phosphoric acid diesters during the hydrolysis. Finally, the cytotoxicity with the MG-63 osteoblast cell lines and proved low cell toxicity from the hydrogels with no significant cell adherence towards the gels similar to PEG-based hydrogels. In summary, this work proves PMEP-hydrogels as degradable alternatives to PEG-hydrogels with similar hydrophilicity and low cell adhesion, which might be used in further tissue engineering and to prevent polymer accumulation.

中文翻译：

---

聚（甲基亚乙基磷酸盐）水凝胶：PEG-水凝胶的可降解和细胞排斥替代品

摘要 合成了一种可降解的水溶性聚磷酸酯（PPE），即聚（甲基亚乙基磷酸酯）-二甲基丙烯酸酯（PMEP-DMA），并通过紫外线照射交联制备了PPE-水凝胶。在用额外的 0.2 wt% 的光引发剂进行紫外线照射后，制备了含有 10 wt% 和 15 wt% PMEP 的水凝胶。研究了无色透明 PPE 水凝胶的溶胀和吸水率。流变分析证明了它们的粘弹性行为。将 PPE 水凝胶与由具有相似聚合度的 PEG-大分子单体制备的聚 (乙二醇) (PEG) 水凝胶进行了比较。水解实验证明，与 PEG 类似物相比，PPE 水凝胶能够成功分解；检测到与 15 wt% 的水凝胶相比，含 10 wt% PMEP 的水凝胶的重量损失更快。核磁共振光谱进一步证明了可溶性 PPE 从网络中释放出来，并在水解过程中形成了磷酸二酯。最后，MG-63 成骨细胞系的细胞毒性证明了水凝胶的低细胞毒性，与基于 PEG 的水凝胶类似的凝胶没有明显的细胞粘附。总之，这项工作证明 PMEP-水凝胶是 PEG-水凝胶的可降解替代品，具有相似的亲水性和低细胞粘附性，可用于进一步的组织工程和防止聚合物积累。MG-63 成骨细胞系的细胞毒性，并证明水凝胶的细胞毒性较低，与基于 PEG 的水凝胶类似的凝胶没有明显的细胞粘附。总之，这项工作证明 PMEP-水凝胶是 PEG-水凝胶的可降解替代品，具有相似的亲水性和低细胞粘附性，可用于进一步的组织工程和防止聚合物积累。MG-63 成骨细胞系的细胞毒性，并证明水凝胶的细胞毒性较低，与基于 PEG 的水凝胶类似的凝胶没有明显的细胞粘附。总之，这项工作证明 PMEP-水凝胶是 PEG-水凝胶的可降解替代品，具有相似的亲水性和低细胞粘附性，可用于进一步的组织工程和防止聚合物积累。