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Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay
Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-10-15 , DOI: 10.1016/j.diagmicrobio.2020.115238
Maryza Graham , Eloise Williams , Nicole Isles , Eka Buadromo , Tebuka Toatu , Julian Druce , Mike Catton , Chantel Lin , Benjamin P. Howden , Deborah A. Williamson

The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had ‘neat’ Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.



中文翻译:

CepheidXpert®Xpress SARS-CoV-2分析中的样品合并

COVID-19大流行对测试所需的实验室用品提出了前所未有的全球需求。实验室已对样品池进行了研究,以此作为保持测试能力的策略。我们评估CepheidXpert®Xpress SARS-CoV-2 RT-PCR分析在4和6样品池中测试样品的性能。使用含有SARS-CoV-2的临床样品,并使用阴性临床样品创建样品池。临床样本的“Xpert®E”基因周期“阈值”整齐,介于20到28之间,当包含在4个或6个样本池中时,所有这些样本均被定性检测。对于这些样品,在4个样品池中,池阈值的中值变化为2.0;在6个样品池中,池的阈值变化为2.9。使用CepheidXpert®Xpress SARS-CoV-2 RT-PCR分析,

更新日期:2020-11-09
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