Immunotherapy is a powerful approach for oncotherapy that relies on enhancing the ability of the immune system to recognize and attack tumors. Effectively provoking the immune effector cells to respond to cancer cells is still a challenge. Here, we present a novel kind of γδ T cell-stimulating hydrogel particle loaded with zoledronate for breast cancer immunotherapy. The hydrogel particles with interconnected nanopores were formed by negatively replicating silica colloidal crystal beads (SCCBs) through using N-isopropylacrylamide (NIPAM) pregel solution doped with black phosphorus quantum dots (BPQDs). Due to the photothermal-responsive property of the BPQDs doped pNIPAM hydrogel, the releasing of zoledronate from these particles could be accurately controlled by using deep penetrating near-infrared (NIR) light. Owing to the direct anti-tumor activity and the capacity of stimulating γδ T-cell to attack tumor cells, our BPQDs-doped pNIPAM hydrogel particles show great potential for γδ T cell activation and proliferation with high therapeutic efficacy for in vivo breast cancer treatment. Thus, the γδ T cell-stimulating hydrogel particles provide a novel clinical treatment option for cancer immunotherapy.

免疫疗法是癌疗法的一种有效方法，它依赖于增强免疫系统识别和攻击肿瘤的能力。有效激发免疫效应细胞对癌细胞的反应仍然是一个挑战。在这里，我们提出了一种新型的负载唑来膦酸盐的γδT细胞刺激水凝胶颗粒，用于乳腺癌的免疫治疗。通过使用掺杂有黑磷量子点（BPQD）的N-异丙基丙烯酰胺（NIPAM）预凝胶溶液通过负复制二氧化硅胶体晶体珠（SCCB）来形成具有互连纳米孔的水凝胶颗粒。由于BPQD掺杂的pNIPAM水凝胶的光热响应特性，唑来膦酸盐从这些颗粒中的释放可以通过使用深穿透近红外（NIR）光来精确控制。由于直接的抗肿瘤活性和刺激γδT细胞攻击肿瘤细胞的能力，我们的BPQDs掺杂的pNIPAM水凝胶颗粒显示了γδT细胞活化和增殖的巨大潜力，对体内乳腺癌的治疗具有很高的治疗功效。因此，刺激γδT细胞的水凝胶颗粒为癌症免疫疗法提供了新的临床治疗选择。