The aim of this study was to evaluate the fertilizing capacity of frozen or vitrified stallion sperm after assessing different warming procedures. In Experiment 1, different warming procedures were compared after sperm vitrification: immersion in extender at 43 °C (C), or in a water bath at 37 °C/30 s (W37), 43 °C/10 s (W43) or 60 °C/5 s (W60). With the W60 treatment, there were greater values (P < 0.05) for VCL (83.93 ± 3.6 μm/s) and ALH (3.00 ± 0.2 μm) than freezing and with the C group, and greater values (P < 0.001) for PM (35.33 ± 2.5 %) than with the W43 treatment. In Experiment 2, the fertilizing capacity of vitrified and frozen sperm was assessed utilizing heterologous IVF procedures, using cattle oocytes. Vitrification resulted in greater values (P < 0.05) than freezing for the number of bound sperm (1.36 ± 0.3 and 0.69 ± 0.2, respectively). There were no differences between frozen or vitrified sperm in pronuclear formation (26 hours post-insemination - hpi; 14.08 ± 4.2 % and 22.78 ± 4.8 %, respectively) or cleavage rate (32.77 ± 4.3 % and 39.66 ± 4.6 %, respectively). In conclusion, vitrified stallion sperm warmed in a water bath at 60 ºC had the capacity to penetrate cattle oocytes, leading to pronuclear formation and hybrid embryo cleavage after heterologous IVF.

本研究的目的是在评估不同的加热程序后评估冷冻或玻璃化种马精子的受精能力。在实验 1 中，比较了精子玻璃化后不同的加温程序：浸入 43 °C (C) 的稀释剂中，或浸入 37 °C/30 s (W37)、43 °C/10 s (W43) 或60 °C/5 秒 (W60)。W60 治疗组VCL (83.93 ± 3.6 μm/s) 和 ALH (3.00 ± 0.2 μm) 的值（P < 0.05）高于冷冻和 C 组，PM 的值（P < 0.001）更大（P < 0.001） (35.33 ± 2.5 %) 比 W43 处理。在实验 2 中，使用牛卵母细胞，利用异源 IVF 程序评估玻璃化和冷冻精子的受精能力。玻璃化导致更大的值（P< 0.05) 而不是冷冻结合精子的数量（分别为 1.36 ± 0.3 和 0.69 ± 0.2）。冷冻或玻璃化精子在原核形成（受精后 26 小时 - hpi；分别为 14.08 ± 4.2 % 和 22.78 ± 4.8 %）或卵裂率（分别为 32.77 ± 4.3 % 和 39.66 ± 4.6 %）方面没有差异。总之，在 60 ºC 水浴中加热的玻璃化种马精子具有穿透牛卵母细胞的能力，导致异源试管婴儿后原核形成和杂交胚胎分裂。