MAIN CONCLUSION Higher vacuolar proton pump activity may increase plant energy and nutrient use efficiency and provide the nexus between plant inoculation with Herbaspirillum seropedicae and growth promotion. Global change and growing human population are exhausting arable land and resources, including water and fertilizers. We present inoculation with the endophytic plant-growth promoting bacterium (PGPB) Herbaspirillum seropedicae as a strategy for promoting growth, nutrient uptake and photosynthetic efficiency in rice (Oryza sativa L.). Because plant nutrient acquisition is coordinated with photosynthesis and the plant carbon status, we hypothesize that inoculation with H. seropedicae will stimulate proton (H+) pumps, increasing plant growth nutrient uptake and photosynthetic efficiency at low nutrient levels. Plants were inoculated and grown in pots with sterile soil for 90 days. Herbaspirillum seropedicae endophytic colonization was successful and, as hypothesized, inoculation (1) stimulated root vacuolar H+ pumps (vacuolar H+-ATPase and vacuolar H+-PPase), and (2) increased plant growth, nutrient contents and photosynthetic efficiency. The results showed that inoculation with the endophytic bacterium H. seropedicae can promote plant growth, nutrient uptake and photosynthetic efficiency, which will likely result in a more efficient use of resources (nutrients and water) and higher production of nutrient-rich food at reduced economic and environmental costs.

中文翻译：

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接种内生菌 Herbaspirillum seropedicae 促进水稻生长、养分吸收和光合效率

主要结 全球变化和不断增长的人口正在耗尽可耕地和资源，包括水和肥料。我们提出了接种内生植物促生长细菌 (PGPB) Herbaspirillum seropedicae 作为促进水稻 (Oryza sativa L.) 生长、养分吸收和光合效率的策略。因为植物养分的获取与光合作用和植物碳状态相协调，我们假设接种 H. seropedicae 会刺激质子 (H+) 泵，在低养分水平下增加植物生长的养分吸收和光合效率。将植物接种并在具有无菌土壤的盆中生长 90 天。Herbaspirillum seropedicae 内生定殖是成功的，并且正如假设的那样，接种 (1) 刺激根液泡 H+ 泵（液泡 H+-ATP 酶和液泡 H+-PPase），以及 (2) 增加植物生长、养分含量和光合效率。结果表明，接种内生细菌 H. seropedicae 可以促进植物生长、养分吸收和光合效率，这可能会导致更有效地利用资源（养分和水）并在降低经济成本的情况下提高营养丰富的食物产量。和环境成本。接种 (1) 刺激根液泡 H+ 泵（液泡 H+-ATPase 和液泡 H+-PPase），以及 (2) 增加植物生长、养分含量和光合效率。结果表明，接种内生细菌 H. seropedicae 可以促进植物生长、养分吸收和光合效率，这可能会导致更有效地利用资源（养分和水）并在降低经济成本的情况下提高营养丰富的食物产量。和环境成本。接种 (1) 刺激根液泡 H+ 泵（液泡 H+-ATPase 和液泡 H+-PPase），以及 (2) 增加植物生长、养分含量和光合效率。结果表明，接种内生细菌 H. seropedicae 可以促进植物生长、养分吸收和光合效率，这可能会导致更有效地利用资源（养分和水）并在降低经济成本的情况下提高营养丰富的食物产量。和环境成本。