The aim of this study was to investigate the biohydrogen production from thermal (T), alkaline (A) or thermal-alkaline (TA) pretreated sludge fermentation liquid (SFL) in a microbial electrolysis cells (MECs) without buffer addition. Highest hydrogen yield of 36.87±4.36 mgH₂/gVSS (0.026 m³/kg COD) was achieved in TA pretreated SFL separated by centrifugation, which was 5.12, 2.35 and 43.25 times higher than that of individual alkaline, thermal pretreatment and raw sludge, respectively. Separating SFL from sludge by centrifugation eliminated the negative effects of particulate matters, was more conducive for hydrogen production than filtration. The accumulated short chain fatty acid (SCFAs) after pretreatments were the main substrates for MEC hydrogen production. The maximum utilization ratio of acetic acid, propionic acid and n-butyric acid was 93.69%, 90.72% and 91.85%, respectively. These results revealed that pretreated WAS was highly efficient to stimulate the accumulation of SCFAs. And the characteristics and cascade bioconversion of complex substrates were the main factor that determined the energy efficiency and hydrogen conversion rate of MECs.

这项研究的目的是研究在不添加缓冲液的情况下，在微生物电解池（MEC）中由热（T），碱（A）或热碱（TA）预处理的污泥发酵液（SFL）产生的生物氢。最高氢气产量36.87±4.36 mgH ₂ / gVSS（0.026 m ³通过离心分离的TA预处理SFL达到了每千克COD），分别比单独的碱性，热预处理和原始污泥分别高出5.12、2.35和43.25倍。通过离心从污泥中分离SFL消除了颗粒物的负面影响，比过滤更有利于制氢。预处理后积累的短链脂肪酸（SCFA）是产生MEC氢的主要底物。乙酸，丙酸和正丁酸的最大利用率分别为93.69％，90.72％和91.85％。这些结果表明，预处理的WAS可以有效刺激SCFA的积累。